| 曹金一,雷 露,段佳林,胡咏武,乔 逸,文爱东,杨志福.黄芪红花配伍对大鼠脑缺血后血管新生及Cav-1/VEGF信号通路的影响[J].现代生物医学进展英文版,2019,19(6):1016-1019. |
| 黄芪红花配伍对大鼠脑缺血后血管新生及Cav-1/VEGF信号通路的影响 |
| Effect of Huangqi-honghua Combination on the Angiogenesis and Cav-1/VEGF Signal Pathway in Brain Tissue of Rats with Cerebral Ischemia Reperfusion Injury |
| Received:September 20, 2018 Revised:October 13, 2018 |
| DOI：10.13241/j.cnki.pmb.2019.06.004 |
| 中文关键词: 黄芪 红花 脑缺血再灌注 血管新生 小凹蛋白1 血管内皮生长因子 |
| 英文关键词: Huangqi Honghua Ischemia reperfusion injury Angiogenesis Caveolin-1 Vascular endothelial growth factor |
| 基金项目:国家自然科学基金项目（81503280，81573549） |
| Author Name | Affiliation | E-mail | | CAO Jin-yi | Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | 187266749@qq.com | | LEI Lu | School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China | | | DUAN Jia-lin | Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | HU Yong-wu | Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | QIAO Yi | Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | WEN Ai-dong | Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | | | YANG Zhi-fu | Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China | |
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| 中文摘要: |
| 摘要 目的：黄芪红花配伍是否通过调节小凹蛋白1（Caveolin-1，Cav-1）/血管内皮生长因子（vascular endothelial growth factor，VEGF）通路促进血管新生保护大鼠脑缺血损伤。方法：60只雄性SD大鼠随机分为5个组：对照组（Sham组，n=12），模型组（MACO组，n=12），黄芪红花40：1组（n=12），20：1组（n=12），5：1（n=12）。大鼠脑缺血再灌注损伤模型采用尼龙线栓法制作，连续给药21d后，评价神经功能学评分，计算脑梗死体积，采用免疫组化法测定皮质区的微血管密度，采用RT-PCR法检测皮质区VEGF mRNA和Cav-1 mRNA表达，采用Western-blotting法测定皮质区VEGF和Cav-1的蛋白表达。结果：连续给药21d后，各组大鼠的神经功能学评分均有所降低，3个不同比例的黄芪红花组的神经功能学评分降低最为明显（P＜0.01），脑梗死体积较模型组显著减少（P＜0.05~ P＜0.01），微血管密度、VEGF和Cav-1 mRNA和蛋白表达水平均较模型组明显升高（P＜0.05~ P＜0.01）。结论：黄芪红花配伍可能通过调节Cav-1/VEGF 信号通路促进脑缺血再灌注损伤大鼠脑内的血管新生，从而减轻脑缺血损伤，且最佳的配伍比例为黄芪红花5：1。 |
| 英文摘要: |
| ABSTRACT Objective: To explore whether Huangqi-Honghua combination protect brain from cerebral ischemia reperfusion injury through promoting angiogenesis by inducing the Cav-1/VEGF (Caveolin-1/vascular endothelial growth factor) signal pathway. Methods: Sixty rats were randomly divided into five groups: control group, model group and Huangqi-Honghua 40:1, 20:1, 5:1groups（12 rats per group）. The rat cerebral ischemia reperfusion injury models were induced by nylon monofilament. After 21 days of administration, the neurological scores were evaluated, the infarct volumes were calculated. Immunohistochemical method was used to detect the microvessel density (MVD) in brain tissue. RT-PCR and Western-blotting was used to detect the mRNA and protein expression levels of Cav-1 and VEGF. Results: After 21 days of administration, the neurological scores of each group were decreased, especially the Huangqi-Honghua groups （P＜0.01）, and infarct volume （P＜0.05~ P＜0.01） in Huangqi-Honghua groups were significantly lower than the model group. Compared with model group, the level of MVD, Cav-1 and VEGF mRNA and protein expression levels were significantly higher in Huangqi-Honghua treatment group（P＜0.05~ P＜0.01）. Conclusion: Huangqi-Honghua combination has protective against cerebral ischemia injury, the Cav-1/VEGF pathway may be involved and then promote the angiogenesis. Additionally, the best proportion of Huangqi-Honghua was 5:1. |
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