| 王少鹏,赵红艳,安晶涛,孙婷婷,唐 林.生长激素对实验大鼠牙齿移动过程中VEGF表达的影响[J].现代生物医学进展英文版,2019,19(3):445-448. |
| 生长激素对实验大鼠牙齿移动过程中VEGF表达的影响 |
| The Effect of Growth Hormone on the Expression of VEGF during the Tooth Movement of Experimental Rats |
| Received:April 27, 2018 Revised:May 23, 2018 |
| DOI：10.13241/j.cnki.pmb.2019.03.010 |
| 中文关键词: 生长激素 牙齿移动 VEGF 正畸 |
| 英文关键词: Growth hormone Tooth movement VEGF Orthodontics |
| 基金项目:黑龙江省博士后科研启动基金项目（LBH-Q11046）；黑龙江省卫计委指导项目（2007-264） |
| Author Name | Affiliation | E-mail | | WANG Shao-peng | Department of Orthodontics, College of Stomatology, Harbin Medical University, Harbin, Heilongjiang, 150001, China | 314195800@qq.com | | ZHAO Hong-yan | Department of Orthodontics, College of Stomatology, Harbin Medical University, Harbin, Heilongjiang, 150001, China | | | AN Jing-tao | Department of Orthodontics, College of Stomatology, Harbin Medical University, Harbin, Heilongjiang, 150001, China | | | SUN Ting-ting | Department of Orthodontics, College of Stomatology, Harbin Medical University, Harbin, Heilongjiang, 150001, China | | | TANG Lin | Department of Orthodontics, College of Stomatology, Harbin Medical University, Harbin, Heilongjiang, 150001, China | |
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| 中文摘要: |
| 摘要 目的：探讨生长激素（growth hormone,GH）对实验大鼠牙齿移动过程中血管内皮生长因子（vascular endothelial growth factor,VEGF）在牙周组织中表达的影响。方法：将40只7周龄大的雄性Wistar大鼠根据是否注射生长激素分为实验组（E）和对照组（C）。将50 g力值加于近中左侧上颌第一磨牙。E组和C组分别腹部皮下注射GH（0.15IU/公斤/天）及等剂量的生理盐水。大鼠分别在第1、3、7、14和21天处死。上颌第一磨牙及其牙周组织切片行VEGF免疫组织化学染色，并进行图像分析和统计。结果：无论张力侧及压力侧VEGF在实验组表达的量均高于对照组，第3天组中张力侧及压力侧实验组平均光密度值分别为174.47±7.53和345.80±25.46与其各自对照组相比较数据具有统计学意义（P<0.05），两侧实验组的VEGF的表达强度峰值均出现在第7天，分别为669.97±3.22和923.77±18.41差异具有统计学意义（P<0.05），VEGF表达峰值及峰值之前压力侧无论实验组和对照组VEGF的表达强度要明显高于张力侧相应分组。结论：短期注射生长激素促进了牙周组织中VEGF的表达，对于正畸牙齿移动具有一定的促进作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of growth hormone (GH) on VEGF expression during orthodontic tooth movement in experimental rats. Methods: 40 male Wistar rats with 7 weeks old were divided into experimental groups(E) and control groups(C)based on whether the growth hormone was injected, adding the 50 g force to upper left maxillary first molar. Group E and C were injected subcutaneously with GH( 0.15 IU/kg/d) and equal does of saline. Rats were killed on 1, 3, 7, 14 and 21 days respectively. Sections of maxillary first molar and periodontal tissue were prepared for immunohistochemical staining of VEGF, then being proceeded with the image analysis and statistics. Results: The expression level of VEGF in the experimental group was higher than that in the control group. In the third day group, the mean optical density values of the tension side and the pressure side of experimental group were respectively 174.47±7.53 and 345.80±25.46, compared with their control group, the data were statistically significant (P<0.05). The peak expression intensity of VEGF in the two experimental groups appeared in the 7th day, the dates were respectively 669.97±3.22 and 923.77±18.41, the difference had significant statistical significance (P<0.05). The expression of VEGF in the experimental group and the control group was significantly higher than that of the tension side groupin the peak and prior to the peak ofthe VEGF expression. Conclusion: The short-term injection growth hormone promoted the expression of VEGF in periodontal tissues，and it has certain promoting effect on orthodontic tooth movement. |
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