| 赵 强,陈文娟,韩 乐,廖 红,王景昌.长链非编码RNA FOXO1在肝癌中的表达及意义[J].现代生物医学进展英文版,2018,(24):4712-4715. |
| 长链非编码RNA FOXO1在肝癌中的表达及意义 |
| Expression and Significance of Long Non-coding RNA FOXO1 in Hepatocellular Carcinoma |
| Received:March 06, 2018 Revised:March 31, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.24.024 |
| 中文关键词: 长链非编码RNA lncFOXO1 原发性肝癌 实时荧光定量PCR |
| 英文关键词: Long non-coding RNA LncFOXO1 Hepatocellular carcinoma qRT-PCR |
| 基金项目:陕西省卫计委卫生科研基金项目（2016E003） |
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| 中文摘要: |
| 摘要 目的：探讨长链非编码RNA LncFOXO1(Forkhead transcription factor O1)在原发性肝癌中的表达及意义。方法：收集临床上手术切除的肝癌组织及相应的癌旁组织并提取组织总RNA并反转录为cDNA；采用用实时荧光定量PCR技术检测lncFOXO1在76例肝癌及相应癌旁组织标本中的表达，并分析其表达水平与肝癌患者临床病理参数之间的关系。结果：lncFOXO1在原发性肝癌组织中表达水平明显低于癌旁组织，差异有统计学意义(P<0.05)，肝癌组织中lncFOXO1的表达与肿瘤的大小、肿瘤的个数、组织学分级和TNM分期相关(P<0.05)，而与性别、年龄、HBV和AFP不相关(P>0.05)。结论：lncFOXO1在肝癌组织中呈低表达并与肝癌患者的临床病理参数相关，其可能参与肝癌的发生发展过程。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression and clinical significance of lncFOXO1 in the hepatocellular carcinoma. Methods: 76 cases of HCC specimens and paired adjacent normal liver tissues (ANLTs) after surgery were collected. qRT-PCR was performed to measure the expression level of lncFOXO1 in 76 HCC and matched ANLTs. The relationship between lncFOXO1 expression and the clinical pathological parameters of patients were analyzed. Results: The expression of lncFOXO1 in HCC tissues was significantly lower than in ANLTs(P<0.05). The expression of lncFOXO1 in HCC tissues showed no obvious correlation with the age, gender, HBV and AFP level of HCC patients(P>0.05). However, lncFOXO1 expression was obviously correlated with the tumor size and number, histolog- ical grade, TNM stage of HCC(P<0.05). Conclusion: lncFOXO1 was down-regulated in HCC tissues and correlated with the clinico- pathological characteristics of HCC, it may participate in the development and progression of HCC. |
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