| 施 恩,张 毅,田少奇,刘英芹,赵 峡.羧甲基壳聚糖促进小鼠皮肤毛囊再生修复的组织形态学研究[J].现代生物医学进展英文版,2018,(24):4614-4622. |
| 羧甲基壳聚糖促进小鼠皮肤毛囊再生修复的组织形态学研究 |
| Study of the Effect of Carboxymethyl Chitosan in Promoting the Regeneration of Mice Skin Hair Follicle with the Tissue Morphology |
| Received:March 27, 2018 Revised:April 24, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.24.003 |
| 中文关键词: 羧甲基壳聚糖 小鼠 皮肤毛囊 再生修复 组织形态学 |
| 英文关键词: Carboxymethyl chitosan Mice Skin hair follicle Regeneration repair Tissue morphology |
| 基金项目:国家自然科学基金项目（81660304） |
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| 中文摘要: |
| 摘要 目的：观察羧甲基壳聚糖(CMC)促进小鼠皮肤毛囊再生修复的组织形态学结构变化，并与新生小鼠皮肤毛囊的组织形态学结构对比。方法：通过切除皮肤组织建立小鼠背部皮肤组织全层缺损模型，随机分为4组：CMC组、清得佳组、生理盐水组与空白对照组，除空白对照组外其余3组每天分别连续给药，第21天处死；为评估造模后小鼠皮肤毛囊组织的再生修复程度，另取新生小鼠36只，每日处死3只；应用Image J技术观察并测量各组小鼠皮肤组织创面修复率；HE染色后光镜下观察新生小鼠皮肤组织厚度、毛囊及“腺泡样”结构数量；对比分析各组小鼠模型第21天和新生小鼠皮肤毛囊的组织形态学结构。结果：第3 天和17 天各组小鼠模型皮肤创面再生修复率：CMC组为33.07±9.52%和96.75±2.11%，清得佳组为24.18±6.14%和92.22±0.62%，生理盐水组为21.06±11.59%和87.38±2.79%，空白对照组为8.66±2.27%和85.15±1.34%；采用线性模型重复度量方差分析法，对CMC组与清得佳组、生理盐水组和空白对照组小鼠皮肤创面第17天再生修复率比较存在显著性差异（F=11.970，17.666，8.828，P＜0.05）。新生小鼠皮肤厚度、毛囊及“腺泡样”结构数量随小鼠日龄增长而增加（r=0.983，0.922，P＜0.05）。与新生小鼠皮肤毛囊的组织形态学结构相比，第21天CMC组、清得佳组、生理盐水组及空白对照组小鼠模型皮肤组织再生修复程度分别相当于新生小鼠皮肤毛囊发育后期、中期、初期及瘢痕组织。结论：CMC具有促进皮肤毛囊组织形态学结构再生修复的重要作用。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the effect of the carboxymethyl chitosan (CMC) in promoting the regenerative repair of mice skin hair follicle and compare with the tissue morphology of newborn mice skin. Methods: First to establish the mice model of full-thick- ness skin defects. Then the mice models were divided into 4 groups: CMC group, Qing dejia group, NaCl group, and the blank Control group. There were 10 mice in each group. Except the mice in blank Control group, medicines were given to the mice in the other three groups accordingly until 21 day. Thirty-six newborn mice were also included into the study. The repair rate of the mice model with skin hair follicle and the development of the newborn mice skin were measured with the Image J technique. The development of the skin hair follicle was observed with HE staining technique. The regeneration repaired morphological structures of the mice models executed in the 21 day in the four groups were compared with the skin tissue morphology of newborn mice. Results: The regenerative repair rates of the skin defect at 3 and 17 day were 33.07±9.52% and 96.75±2.11% in CMC group, 24.18±6.14% and 92.22±0.62% in Qingdejia group, 21.06±11.59% and 87.38±2.79% in NaCl group, and 8.66±2.27% and 85.15±1.34% in blank Control group respectively. Compar- ing the rates in CMC group with those in the other three groups respectively, significant differences were found（F=11.970, 17.666, 8.828, P＜0.05）. The development of skin thickness and the hair follicle number increase with the growth day age of newborn mice（r=0.983, 0.922, P＜0.05）. Compared with the skin tissue morphology of newborn mice, the structure of the regeneration repaired skin at 21 day in CMC group, Qingdejia group, NaCL group and blank Control group equalized the late-stage of development, the mid-stage of develop- ment, the early-stage of development and the stage of scar respectively. Conclusion: CMC may play an important role in promoting skin regenerative repair. |
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