| 吴利英,双 婷,谢婷婷,李玲霞,白 璐.细胞因子诱导杀伤细胞对卵巢癌细胞耐药的影响[J].现代生物医学进展英文版,2018,(23):4405-4408. |
| 细胞因子诱导杀伤细胞对卵巢癌细胞耐药的影响 |
| Effect of Cytokine-induced Killer Cells on the Drug Resistance of Ovarian Cells |
| Received:April 30, 2018 Revised:May 26, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.23.002 |
| 中文关键词: 细胞因子诱导杀伤细胞 卵巢癌 耐药 糖类抗原125 人附睾蛋白4 |
| 英文关键词: CIK Ovarian cancer Drug-resistant CA125 HE-4 |
| 基金项目:国家自然科学基金项目（81702555） |
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| 中文摘要: |
| 摘要 目的：探究细胞因子诱导杀伤细胞(cytokine-induced killers，CIK)对卵巢癌细胞耐药的影响。方法：选取荷人卵巢癌SKOV3／CDDP耐药细胞株，经培养后将其调整为细胞浓度为1×105/L的单细胞悬液，均分为2份，分别设定为实验组和对照组，其中对照组给予贝伐单抗，实验组加入CIK进行刺激，两组培养时间均为48 h，而后使用电镜观察细胞形态，并记录细胞凋亡率，而后吸取培养液上层清液，离心后采用放射免疫分析法测定其细胞因子转化生长因子-α(TGF-α)、肿瘤坏死因子-α(TNF-α)的浓度，采用电化学发光法检测糖类抗原125(CA125)及人附睾蛋白4(HE 4)的水平。结果：治疗前，两组细胞形态较为饱满、圆润，无凋亡细胞；治疗后，实验组出现明显细胞萎缩、膜发泡，且出现较多凋亡细胞，实验组细胞凋亡率高于对照组, 实验组早期和中晚期凋亡率均显著高于对照组（P<0.05）。治疗前，两组细胞上清TGF-α、TNF-α、CA125、HE4水平比较差异无统计学意义(P>0.05)；治疗后，实验组细胞上清TGF-α、TNF-α、CA125、HE4水水平均显著低于对照组，差异具有统计学意义(P<0.05)。结论：较贝伐单抗而言，CIK对卵巢癌耐药细胞具有更强的杀伤作用，能够显著降低CA125、HE4水平。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of cytokine induced killer cell line cytokine-induced killer cells(CIK) on the re- sistance of ovarian cancer cells. Methods: Human ovarian cancer SKOV3/CDDP resistant cell line was selected and cultured into a single cell suspension with cell concentration of 1×105/L. The cells were divided into the experimental group and control group, and control group as blank control. The experimental group was stimulated with CIK. The cell morphology of the two groups was observed by elec- tron microscope, the apoptosis rate was recorded, and then the supernatant of the culture medium was extracted. After centrifugation, the concentrations of TGF-α and TNF-α of TGF-α and TNF-α were measured by radioimmunoassay. The levels of tumor markers carbohy- drate antigen 125 (CA125) and human epididymal protein 4 (HE4) in the two groups were determined and compared by electrochemilu- minescence. Results: Before treatment, the morphology of the cells in the two groups was relatively full, round and no apoptotic cells was observed by electron microscope. After treatment, the cells in experimental group were obviously atrophied, and the membrane was foaming. More apoptotic cells appeared in the experimental group and the apoptotic rate in the experimental group was higher than that in the control group. The early and late apoptotic rates in the experimental group were significantly higher than those in the control group (P<0.05). The level of TGF-α, TNF-α in the experimental group were significantly lower than those in the control group before treat- ment(P<0.05), but the level of TGF-α, TNF-α in the experimental group was significantly lower than that in the control group(P<0.05). There was no significant difference between the two groups before treatment(P>0.05). After treatment, the level of CA125 and HE4 in the experimental group were significantly lower than those in the control group(P<0.05). Conclusion: CIK has a strong killing effect on ovarian cancer resistant cells and can significantly reduce the levels of CA125 and HE4. |
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