| 李晓燕,张 艳,李 辉,董春萍,吴贵福.富含血小板血浆和脐带干细胞联用对糖尿病足模型lncRNA表达谱的影响[J].现代生物医学进展英文版,2018,(22):4224-4228. |
| 富含血小板血浆和脐带干细胞联用对糖尿病足模型lncRNA表达谱的影响 |
| Effects of Platelet Rich Plasma and Umbilical Cord Stem Cells on the lncRNA Expression Profiles in Diabetic Foot Models |
| Received:April 09, 2018 Revised:May 06, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.22.005 |
| 中文关键词: 富含血小板血浆 脐带干细胞 糖尿病足 lncRNA表达谱 |
| 英文关键词: Platelet rich plasma Umbilical cord stem cells Diabetic foot Expression profiles of lncRNA |
| 基金项目:陕西省自然科学基础研究计划项目(2016JQ8055） |
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| 中文摘要: |
| 摘要 目的：探讨富含血小板血浆(PRP)和脐带干细胞(UBC-MSCs)联用对糖尿病足(DF)模型lncRNA表达谱的影响。方法：采用SPF级SD大鼠(20只)建立糖尿病足模型，随机分为4组，每组5只大鼠。A组注射PBS于DF动物溃疡处，B组采用人PRP注射于DF动物溃疡处，C组采用人UBC-MSCs注射于DF动物溃疡处，D组采用人UBC-MSCs+PRP注射于DF动物溃疡处。治疗14d后，记录大鼠的血糖水平与血清CRP、TNF-α、VEGF相对表达，采用arraystar公司12×135K芯片测定lncRNA的表达谱。结果：治疗14d后，B、C、D组均较A组显著降低，D组显著低于B、C组，组间差异有统计学意义(P<0.05)；D组大鼠的血清CRP、TNF-α相对表达较A、B、C组显著降低，血清VEGF相对表达较A、B、C组显著升高，差异都有统计学意义(P<0.05)。B组与A组比较存在1563个显著差异的IncRNA，C组与A组比较存在1982个显著差异的IncRNA，D组与A组比较存在2854个显著差异的IncRNA。将靶基因带入差异mRNA数据，筛选得到与DF相关的靶基因的lncRNA有6条，具体为CHI3L1、FA2H、CAMTA1、DOCK、STRADB和TNFRSF1A(P1101039、BF74369、Uc001aoh、N385370、AF116618和HMlincRNA1336)。结论：UBC-MSCs和PRP联合可能通过改变lncRNA的表达，影响炎症因子、血管生成因子等的表达，进而发挥降血糖和治疗DF的目的。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of platelet rich plasma (PRP) and umbilical cord stem cells (UBC-MSCs) on the expression profiles of lncRNA in diabetic foot (DF) models. Methods: The diabetic foot model of SPF SD rats (20 rats) were established and were divided into 4 groups randomly, 5 rats in each group. Group A were injected with PBS to DF animal ulcers, Group B were injected with PRP to DF animal ulcer, Group C were injected with UBC-MSCs to DF animal ulcer, Group D were injected with UBC-MSCs+PRP to DF animal ulcer, The expression level of lncRNA were detected by arraystar 12×135K chip. Results: After treatment for 14d, the blood glucose levels in group A, group B, group C and D group were 23.38±2.85 mmol/L, 13.88±3.19 mmol/L, 13.48±2.84 mmol/L and 7.22±3.18 mmol/L respectively, and compared the difference were statistically significant (P<0.05). The relative expression level of CRP and TNF-α in group Dwere decreased significantly, while VEGF were increased significantly, compared the difference were statistically significant (P<0.05). There were 1563 IncRNAs were significant differences compared between group B and group A, so that were 1982IncRNAs and 2854IncRNAs compared between the group C and group A, group D and group A; there were 6 lncRNAs of DF related target genes of common screened DF were changed in the difference mRNA data[CHI3L1, FA2H, CAMTA1, DOCK, STRADB and TNFRSF1A(P1101039, BF74369, Uc001aoh, N385370, AF116618 and HMlincRNA1336)]. Conclusion: The combination of UBC-MSCs and PRP could decrease the blood glucose and treat DF via may changing the expression of lncRNA and further mediating the expression of inflammatory factors, angiogenic factors, etc. |
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