| 任楠楠,郭旭昌,周 磊,周 珊,黄 芳.探讨不同阶段哮喘患儿Gal-3、MPO、GM-CSF、TGF-β1水平在支气管肺泡灌洗液中的变化及临床意义[J].现代生物医学进展英文版,2018,(21):4160-4163. |
| 探讨不同阶段哮喘患儿Gal-3、MPO、GM-CSF、TGF-β1水平在支气管肺泡灌洗液中的变化及临床意义 |
| The Changes and Clinical Significance of Gal-3, MPO, GM-CSF and TGF- beta 1 in Bronchoalveolar Lavage Fluid in Children with Asthma at Different Stages |
| Received:April 25, 2018 Revised:May 20, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.21.036 |
| 中文关键词: 哮喘 儿童 支气管肺泡灌洗液 转化生长因子-β1 半乳糖凝集素-3 粒细胞-巨噬细胞集落刺激因子 髓过氧化物酶 |
| 英文关键词: Asthma Child Bronchoalveolar lavage fluid Gal-3 MPO GM-CSF TGF-β1 |
| 基金项目:陕西省自然科学基础研究计划项目（S2016YFJQ1066） |
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| 中文摘要: |
| 摘要 目的：探讨半乳糖凝集素-3(Gal-3)、髓过氧化物酶（MPO）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和转化生长因子-β1（TGF-β1）在不同阶段哮喘患儿支气管肺泡灌洗液中水平变化及临床指导意义。方法：选择我院2016年3月至2017年6月接诊的114例哮喘患儿（观察组）和36例非哮喘患儿（对照组）的BALF作为研究标本，分别测定细胞总数和分类计数以及Gal-3、MPO、GM-CSF和TGF-β1水平，并进行对比分析。结果：轻度和中重度患儿组与对照组支气管肺泡灌洗液中的TCS、巨噬细胞比较差异无统计学意义（P>0.05），中粒细胞、嗜酸性粒细胞和上皮细胞数差异具有统计学意义（P<0.05）;轻度和中重度组中性粒细胞、嗜酸性粒细胞和上皮细胞数差异具有统计学意义（P<0.05）；观察组患儿支气管肺泡灌洗液Gal-3、MPO、GM-CSF、TGF-β1表达水平高于对照组[3.59±0.49 vs 10.27±3.89]、[13.82±3.54 vs 21.03±7.05]、[123.07±25.68vs154.82±36.44]、[121.68±7.98vs221.59±44.12]；中重度组患儿支气管肺泡灌洗液Gal-3、MPO、GM-CSF、TGF-β1表达水平高于轻度组[6.91±1.43 vs 14.42±3.17]、[15.52±4.42 vs25.18±9.73]、[129.53±30.42vs164.78±34.03]、[171.35±21.48vs240.83±34.08]；不同阶段哮喘患儿的支气管肺泡灌洗液中Gal-3与MPO、GM-CSF和TGF-β1的表达水平之前存在正相关关系。结论：哮喘患儿BALF中Gal-3、MPO、GM-CSF、TGF-β1的水平变化与病情密切相关，可作为监测哮喘病症发展的有效指标。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the level and clinical significance of Gal-3, MPO, GM-CSF and TGF- beta 1 in bronchoalve- olar lavage fluid of children with asthma in different periods. Methods: 114 cases of children with asthma in our hospital from March 2016 to June 2017 were (observation group) and 36 cases of non asthmatic children (control group) BALF as research sample, 1 levels of total cell counts and classification as well as Gal-3, MPO, GM-CSF and TGF-β1were measured and analyzed. Results: Mild and severe HIE group and control group in the bronchoalveolar lavage fluid of TCS macrophages, no significant difference (P>0.05), but in eosinophil and epithelial cell number had significant difference (P<0.05); with statistical significance of neutrophils, moderate and severe group eosinophils and epithelial cell number difference (P<0.05); the observation group with bronchoalveolar lavage fluid Gal-3, MPO, GM-CSF, TGF- beta 1 expression level was higher than the control group [3.59±0.49 vs 10.27±3.89], [13.82±3.54 vs 21.03±7.05], [123.07±25.68vs154.82±36.44], [121.68±7.98vs221.59±44.12] in; severe group with bronchoalveolar lavage fluid Gal-3, MPO, GM-CSF, TGF- beta 1 expression level is higher than that of mild group [6.91±1.43 vs 14.42±3.17], [15.52±vs25.18±9.73], [4.42 129.53±30.42vs164.78±34.03], [171.35 ±21.48vs240.83±34.08]; there was a positive correlation between the levels of Gal-3 and MPO, GM-CSF and TGF-β1 in bronchoalveolar lavage fluid of asthmatic children in different stages. Conclusion: The changes of the levels of Gal-3, MPO, GM-CSF and TGF-β1 in BALF of asthmatic children are closely related to the condition, which can be used as an effective index to monitor the development of asthma. |
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