| 张 立,刘江伟,康 燕,马 娜,唐 军,高晓康,沈才福,董 翔.姜黄素预处理对沙漠干热环境中暑大鼠心肌细胞凋亡和Caspase-3活性的影响[J].现代生物医学进展英文版,2018,(21):4022-4026. |
| 姜黄素预处理对沙漠干热环境中暑大鼠心肌细胞凋亡和Caspase-3活性的影响 |
| The Effects of Curcumin Pretreatment on Myocardial Apoptosis and Caspase-3 Activity of Heatstroke Rats in Dry-heat Environment of Desert |
| Received:May 31, 2018 Revised:July 25, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.21.005 |
| 中文关键词: 姜黄素 干热环境 心肌损伤 凋亡 半胱天冬氨酸蛋白酶-3 |
| 英文关键词: Curcumin Dry heat environment Myocardial injury Apoptosis Caspase-3 |
| 基金项目:军队临床高新技术重大项目(2010gxjs016) |
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| 中文摘要: |
| 摘要 目的：研究姜黄素预处理对沙漠干热环境不同阶段中暑大鼠心肌损伤、细胞凋亡及半胱天冬氨酸蛋白酶-3(Caspase-3)活性的影响。方法：选择雄性健康SD大鼠80只，将其随机分为2组(n=40)：盐水对照组以及姜黄素预处理组。每组再分为4个亚组(n=10)：干热0 min组(即常温组)，干热50 min组(轻度中暑组)，干热100 min组(中度中暑组)，干热150 min组(重度中暑组)。盐水组大鼠给予0.9%生理盐水灌胃，姜黄素组大鼠给予剂量为100 mg/kg姜黄素灌胃，各组大鼠均连续灌胃7天。0 min组置于常温环境中，其余组放置在西北特殊环境人工实验舱内，设置干热环境：温度(41±0.5)℃，湿度(10±1)%，并在相应时间点麻醉大鼠，取血液以及心脏组织。检测血液心肌酶谱磷酸肌酸激酶(CK)、磷酸肌酸激酶同工酶(CK-MB)及乳酸脱氢酶(LDH)水平，用TUNEL法检测心肌细胞凋亡率，Caspase-3比色试剂盒检测Caspase-3活性。结果：随着干热环境时间的延长，大鼠血清CK、CK-MB、LDH水平、心肌细胞凋亡率及心肌组织Caspase-3活性均逐渐升高，而姜黄素预处理组在干热环境放置50 min、100 min和150 min时的血清CK、CK-MB、LDH水平、心肌细胞凋亡率及心肌组织Caspase-3活性均明显低于对照组(P<0.01)。结论：姜黄素预处理可通过减少心肌细胞凋亡、增加Caspase-3活性，保护沙漠干热环境中暑大鼠心肌损伤。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the protective effects of curcumin pretreatment on myocardial injury, apoptosis and Cas- pase-3 activity of different stage heatstroke rats in dry-heat environment of desert. Methods: 80 Sprague-Dawley rats were randomly di- vided into 2 groups (n=40): Saline control group and Curcumin pretreatment group. Each group was divided into four subgroups (n=10): 0min group (normal temperature group), 50 min group（mild heatstoke group）, 100 min group(moderate heatstroke group), 150 min group(severe heatstroke). Rats of the saline group were given 0.9% normal saline, the curcumin group was administered with curcumin, the dose was 100 mg/kg bw, and the rats were administered continuously for 7 days. The rats of 0 min group were put at room temperature, the other groups were transferred to the climate cabin (The Simulated Climate Cabin for Special Environment of Northwest of China) with the conditions of (41.5±0.5)℃ temperature, (10±1) % relative humidity to make the rat heatstroke model of dry heat environment of desert. The rats were anesthetized at the corresponding time points and blood and heart tissues were collected. The serum myocardium zymogram: creatine kinase (CK), isoenzyme of creatine kinase (CK-MB) and lactate dehydrogenase (LDH) were detected, cellular apop- tosis was analyzed by TUNEL assay, Caspase-3 activity were detected by Caspase-3 colorimetric kit. Results: The serum myocardium zy- mogram (CK, CK-MB, LDH), myocardial apoptosis rate and Caspase-3 activity were all increased with the exposed time in the dry heat environment and the progression of the heat stroke. The serum CK, CK-MB and LDH, myocardial apoptosis rate and Caspase-3 activity were significanly lower in curcumin pretreatment group than control group at the 50 min, 100 min and 150 min time point(P<0.01). Conclusion: Curcumin preteatment may through inhibiting the myocardial apoptosis and increasing the Caspase-3 activity to exert protective effects on myocardial injury during heatstroke in dry heat environment of desert. |
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