| 赵浩茜,邹慧玲,胡 芳.有氧运动抑制自发性高血压大鼠心肌中HMGB1/TLR4炎症通路[J].现代生物医学进展英文版,2018,(19):3633-3636. |
| 有氧运动抑制自发性高血压大鼠心肌中HMGB1/TLR4炎症通路 |
| Aerobic Exercise Training Inhibits Cardiac HMGB1/TLR4 Pathway in Spontaneously Hypertensive Rats |
| Received:March 21, 2018 Revised:April 18, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.19.007 |
| 中文关键词: 有氧运动 自发性高血压大鼠 高迁移率族蛋白1 Toll样受体4 心肌纤维化 |
| 英文关键词: Aerobic exercise Spontaneously hypertensive rats High mobility group box-1 protein Toll like receptor 4 Cardiac fibrosis |
| 基金项目: |
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| 中文摘要: |
| 摘要 目的：探讨有氧运动对自发性高血压大鼠心肌中高迁移率族蛋白1 （high mobility group box-1 protein, HMGB1）/Toll样受体4（toll like receptor 4，TLR4）炎症通路的影响。方法：成年雄性大鼠分为三组：同源正常血压大鼠（Wistar-Kyoto rats，WKY），自发性高血压大鼠（spontaneoully hypertensive rat, SHR），自发性高血压大鼠运动组（SHR + exercise，SHR+EX）。运动为中等强度跑台运动，自制跑台，倾斜角设置为10度，运动强度逐步增强，持续12周。尾动脉检测血压，收集左心室，Masson染色检测心肌组织纤维化水平，RT-PCR法检测心肌中纤维化相关基因的mRNA水平，ELISA法检测炎症因子白介素6（Interleukin 6, IL-6）和肿瘤坏死因子α（tumor necrosis factor alpha, TNF-α）的蛋白水平，Western blot法检测HMGB1和TLR4的蛋白表达。结果：有氧运动可以明显降低SHR大鼠心肌中HMGB1（P<0.05）和TLR4（P<0.05）的蛋白表达以及炎症因子IL-6（P<0.05）和TNF-α（P<0.05）的蛋白水平。经过的有氧运动之后，SHR大鼠的体重（P<0.05）、收缩压（P<0.05）、舒张压明显降低（P<0.05）；心肌纤维化水平和心肌中I型胶原（P<0.05）、转化生长因子-β（transforming growth factor-β，TGF-β，P<0.05)、α平滑肌肌动蛋白（α-smooth muscle actin，α-SMA，P<0.05）的mRNA水平出现显著下降。结论：有氧运动可抑制自发性高血压大鼠心肌中HMGB1/TLR4炎症通路。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the role of high mobility group box-1 protein (HMGB1)/toll like receptor 4 (TLR4) pathway in exercise-induced attenuation of cardiac fibrosis in spontaneously hypertensive rats (SHR). Methods: The adult male SHR were divided into three groups as follows: (1) normotensive Wistar-Kyoto rats (WKY); spontaneously hypertensive rats (SHR); (3) SHR received exer- cise (SHR+EX). The aerobic exercise training on treadmill with an incline of 10 degrees was performed for 12 weeks. The intensity of the exercise training was moderate. Twenty-four hours after the last training session, blood pressure was measured, the left ventricles were removed for morphometric analysis, RT-PCR analysis, ELISA and Western blot analysis. Results: In this work, exercise training in SHR suppressed protein expression of HMGB1(P<0.05) and TLR4 (P<0.05), and reduced protein levels of inflammatory cytokines including interleukin 6 (IL-6, P<0.05) and tumor necrosis factor alpha (TNF-α, P<0.05) in myocardium. Furthermore, aerobic exercise training in SHR reduced blood pressure(P<0.05) and body weight (P<0.05), and effectively attenuated fibrosis (P<0.05) and reduced mRNA expres- sion of fibrosis-related proteins including Collagen-I (P<0.05), transforming growth factor-β(TGF-β, P<0.05), and α-smooth muscle actin (α-SMA, P<0.05) in myocardium. Conclusion: Aerobic exercise training ameliorated fibrosis and inflammation in myocardium of SHR, possibly via suppressing HMGB1/TLR4 pathway. |
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