| 王 涛,康 涛,雷 琦,杨 谦,曹冰清.S100a4-Cre×Ddr2flox/flox条件敲除小鼠的繁育及基因型鉴定[J].现代生物医学进展英文版,2018,(19):3628-3632. |
| S100a4-Cre×Ddr2flox/flox条件敲除小鼠的繁育及基因型鉴定 |
| Reproduction and Genotyping of the S100a4-Cre×Ddr2flox/flox Conditional Knock-Out Mice |
| Received:February 28, 2018 Revised:March 27, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.19.006 |
| 中文关键词: Cre-LoxP技术 Ddr2 条件性敲除小鼠 |
| 英文关键词: Cre-LoxP System Ddr2 Conditional Knockout Mice |
| 基金项目:陕西省自然科学基础研究计划项目（2017JM8029）；陕西省社会发展科技攻关项目（2014K11-03-02-07） |
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| 中文摘要: |
| 摘要 目的：利用Cre-LoxP重组酶系统构建成纤维细胞中Ddr2特异性敲除的纯合子小鼠并鉴定，为进一步研究Ddr2在肺纤维化中的作用提供基础。方法：将购买的Ddr2loxp小鼠和成纤维细胞特异表达的S100a4-Cre小鼠分别繁殖与鉴定，然后将两种小鼠杂交与鉴定，最终得到基因型为Cre×Ddr2flox/flox的纯合子小鼠就是在成纤维细胞中Ddr2条件性敲除小鼠。结果：成功繁殖并用PCR技术准确鉴定了基因型为Cre×Ddr2flox/flox的纯合子小鼠，Western blot 结果表明纯合子小鼠的肺成纤维细胞中Ddr2已缺失。结论：本研究利用Cre-LoxP系统成功构建了在成纤维细胞中Ddr2特异性敲除的纯合子小鼠，为进一步研究Ddr2在肺纤维化发展中的机制提供了研究平台。 |
| 英文摘要: |
| ABSTRACT Objective: To generate and identify the fibroblast specific Ddr2 knockout homozygous mice, for the research of the role of the Ddr2 gene on the pulmonary fibrosis. Methods: First, The introduced Ddr2 loxp mice and the S100a4-Cre mice which ex- pressed fibroblast cell-specific had reproduced and genotyped respectively, then the two kinds of mice were crossed, finally the off-spring mice which genotyping CreDdr2flox/flox were the homozygous mice. Results: CreuDdr2flox/flox mice were breed successfully and their geno- type were identified accurately by PCR. Conclusion: We successfully generated fibroblast specific Ddr2 gene knockout homozygous mice through Cre-LoxPtechnology, that can supply the tool for research the role of the Ddr2 gene on pulmonary fibrosis. |
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