| 周思齐,李皓桓,张宇标,施家奇,蔡伟松.甲基莲心碱促进骨肉瘤143B细胞凋亡的相关研究[J].现代生物医学进展英文版,2018,(19):3612-3617. |
| 甲基莲心碱促进骨肉瘤143B细胞凋亡的相关研究 |
| The Effect of Neferine on the Apoptosis of Osteosarcoma 143B Cells |
| Received:April 28, 2018 Revised:May 23, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.19.003 |
| 中文关键词: 甲基莲心碱 骨肉瘤 增殖 凋亡 |
| 英文关键词: Neferine Osteosarcoma Proliferation Apoptosis |
| 基金项目:湖北省自然科学基金项目（ZRMS2017000057） |
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| 中文摘要: |
| 摘要 目的：研究不同浓度甲基莲心碱对骨肉瘤143B细胞增值迁移的影响及其诱导凋亡的相关机制。方法：不同浓度甲基莲心碱处理骨肉瘤143B之后，CCK-8法检测甲基莲心碱对骨肉瘤143B细胞的增值抑制作用；细胞划痕实验检测不同浓度甲基莲心碱对骨肉瘤143B细胞迁移的影响；流式细胞术检测甲基莲心碱对癌细胞的凋亡率和周期分布；Western Blot 检测甲基莲心碱对癌细胞相关蛋白Bcl-2、Bax的表达。结果：CCK-8显示甲基莲心碱能抑制骨肉瘤143B细胞的增值且以浓度和时间依赖的方式(P<0.05)；给药组(20, 40, 60 μmol?L-1)24 h细胞迁移率分别为(62.35±4.15) %, (40.74±4.80) %, (25.10±5.52) %，较对照组(75.89±5.24)%明显降低(P<0.05)；流式细胞术结果显示甲基莲心碱能使骨肉瘤143B细胞周期阻滞于G0/G1期，且呈剂量依赖性诱导癌细胞凋亡(P<0.05)；Western Blot证明甲基莲心碱可促进癌细胞中促凋亡蛋白Bax的表达，而降低抑制凋亡蛋白Bcl-2的表达(P<0.05)。结论：甲基莲心碱可能通过激活线粒体凋亡相关蛋白的表达，发挥抗骨肉瘤143B的作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of different concentrations of neferine on the proliferation, migration and relative mechanism of apoptosis of osteosarcoma 143B cells. Methods: CCK-8 assay was used to detect the the anti-proliferation effect after dif- ferent concentrations of neferine on 143B cells. Wound healing assay was used to detect the effect of neferine on the migration of os- teosarcoma 143B cells. The effect of neferine on cancer cell cycle distribution and apoptosis was analyzed by flow cytometry. Western Blot was used to detect expression of apoptosis-related protein Bax and Bcl-2. Results: CCK-8 assay showed that neferine inhibited the proliferation of osteosarcoma 143B cells in a time- and concentration-dependent manner(P<0.05). The 24-hour cell migration rates of the treated group (20, 40, 60 μmol?L-1) were (62.35±4.15)%, (40.74±4.80)%, (25.10±5.52)%, compared with the control group (75.89±5.24)% were decreased significantly(P<0.05). Flow cytometry showed that the cell cycle of 143B cells were arrested at G0/G1 phase and induced apoptosis of 143B cells at a concentration-dependent manner(P<0.05). Western Blot detection indicated that the expression of pro-apoptotic protein Bax was increased, and anti-apoptotic protein Bcl-2 was decreased in 143B cells when treated with different con- centrations of neferine(P<0.05). Conclusion: Neferine may play a role in anti-osteosarcoma 143B by activating the expression of mito- chondrial apoptosis-related protein. |
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