| 张 勤,肖宗慧,李 丹,万春雷,白宝玲.组蛋白 H3K79同型半胱氨酸修饰位点在神经管畸形中的作用[J].现代生物医学进展英文版,2018,(18):3401-3405. |
| 组蛋白 H3K79同型半胱氨酸修饰位点在神经管畸形中的作用 |
| Role of Histone H3K79 Homocysteinylation in the Neural Tube Defects |
| Received:April 30, 2018 Revised:May 23, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.18.001 |
| 中文关键词: 同型半胱氨酸 组蛋白 H3K79同型半胱氨酸修饰 神经管畸形 |
| 英文关键词: Homocysteine Histone H3K79 homocysteinylation Neural tube defects |
| 基金项目:国家自然科学基金项目（81741044）；北京市自然科学基金项目（7182024） |
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| 中文摘要: |
| 摘要 目的：探讨人类胚胎脑组织中是否存在 H3K79同型半胱氨酸修饰(H3K79Hcy)及其在神经管畸形(NTDs)中的作用。方法：通过质谱检测组蛋白 H3K79是否存在同型半胱氨酸修饰位点。进一步合成包含组蛋白 H3K79位点的同型半胱氨酸(Hcy)修饰的肽段，并与牛血清白蛋白 (BSA)偶联后免疫兔子得到抗组蛋白 H3K79Hcy多克隆抗体，并对抗体进行特异性检测；采用此抗H3K79Hcy抗体比较人类高 Hcy NTDs样本和正常对照样本的 H3K79Hcy水平。结果：①人胚胎组织组蛋白 H3K79位点存在同型半胱氨酸修饰；②高 Hcy水平 NTDs脑组织中 H3K79Hcy修饰水平高于正常对照(P<0.05)。结论：人胚胎组织存在 H3K79Hcy修饰，此修饰异常可能促进神经管畸形的发生。 |
| 英文摘要: |
| ABSTRACT Objective: To verify whether there is H3K79 homocysteinylation (H3K79Hcy) in human embryonic brain tissue and its role in human neural tube defects (NTDs). Methods:Mass spectrometry was used to detect the H3K79Hcy sites in human brain tissue. Further, the peptide containing H3K79Hcy was coupled with bovine serum albumin (BSA). Then the H3K79Hcy peptide-BSA was immunized rabbits to obtain anti H3K79Hcy polyclonal antibody. Then the anti H3k79Hcy antibody specificity was detected. Finally, specific antibody was used to compare the level of H3K79Hcy between high Hcy level human NTDs and normal control samples. Results:(1)The lysine of H3K79 was homocysteinylated in human embryonic brain tissue; (2)The level of H3K79Hcy in the NTDs brain with high Hcy level was found to be higher than that of the normal control (P<0.05). Conclusion:The lysine of H3K79 in human fetal brain was homocysteinylated and the abnormal H3K79Hcy may promote the occurrence of neural tube defects. |
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