| 周思齐,施家奇,蔡伟松,张宇标,李皓桓.缺氧复氧对滑膜细胞IGF-IGFBP-3 以及线粒体的影响[J].现代生物医学进展英文版,2018,(17):3209-3213. |
| 缺氧复氧对滑膜细胞IGF-IGFBP-3 以及线粒体的影响 |
| Effects of Hypoxia and Reoxygenation on IGF-1, IGFBP-3 and Mitochondria in Synovial Cells |
| Received:April 04, 2018 Revised:April 30, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.17.002 |
| 中文关键词: 成纤维样滑膜细胞 IGF-1 IGFBP-3 线粒体 |
| 英文关键词: Fibroblast-likesynoviocyte IGF-1 IGFBP-3 Mitochondria |
| 基金项目:湖北省自然科学基金项目（ZRMS2017000057） |
|
| Hits: 589 |
| Download times: 287 |
| 中文摘要: |
| 摘要 目的：探讨氧波动环境对原代成纤维样滑膜细胞(fibroblast-like synoviocyte, FLS)胰岛素样生长因子-1(insulin growth factor-1, IGF-1)、胰岛素样生长因子结合蛋白-3(insulin-like growth factor binding protein-3, IGFBP-3)及线粒体的影响。方法：分离并鉴定正常人滑膜细胞，再对滑膜细胞进行分组：对照组、缺氧/再充氧(hypoxia/reoxygenation，H/R)干预组。采用实时定量PCR检测滑膜细胞中IGF-1、IGFBP-3的mRNA水平；Western blot 检测滑膜细胞中IGF-1、IGFBP-3的蛋白水平；流式细胞仪检测线粒体膜电位(Mitochondrial membrane potential, MMP)以及线粒体通透性转换孔(Mitochondrial Permeability Transition Pore, MPTP)的变化。结果：与对照组比较，H/R干预组的相对IGF-1和IGFBP-3的mRNA水平和蛋白表达水平显著升高(P<0.05)，膜电位水平降低(P<0.05)，线粒体通透性转换孔开放。结论：氧波动环境可促进IGF-1和IGFBP-3的表达及细胞线粒体损伤，其可能是骨关节炎(OA)发病的重要机制之一。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of oxygen-induced fluctuations on the expression of insulin-like growth factor-1 (IGF-1), insulin-like growth factor binding protein-3 -3, IGFBP-3 and the change of mitochondria. Methods: Synovial cells were isolated and identified, which were divided into control group and Hypoxia/ Reoxygenation (H/R) intervention group. The mRNA levels of IGF-1 and IGFBP-3 in synoviocytes were detected by real-time quantitative PCR; The protein levels of IGF-1 and IGFBP-3 in synoviocytes were detected by Western blot; The mitochondrial membrane potential and mitochondrial permeability transition pore was detected by flow cytometry. Results: Compared with the control group, the mRNA and protein levels of IGF-1 and IGFBP-3 in the H/R intervention group were significantly increased(P<0.05); the membrane potential was significantly lower than that in the control group (P<0.05) and openness of mitochondrial permeability transition pore. Conclusion: The fluctuating environment of oxygen can promote the expression of IGF-1 and IGFBP-3 and the mitochondrial damage, which would be a possible mechanism of osteoarthritis(OA). |
|
View Full Text
View/Add Comment Download reader |
| Close |
