| 吕 娟,曹兰秀,赵 娴,王 江,马 莉.不同剂量朱砂七总蒽醌对H22荷瘤小鼠免疫功能及抗氧化能力的影响[J].现代生物医学进展英文版,2018,(15):2851-2854. |
| 不同剂量朱砂七总蒽醌对H22荷瘤小鼠免疫功能及抗氧化能力的影响 |
| Effects of Different Doses of Total Anthraquinone From Zhushaqi on Immune Function and Antioxidant Capacity in H22 Tumor Bearing Mice |
| Received:December 27, 2017 Revised:January 23, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.15.010 |
| 中文关键词: 朱砂七总蒽醌 H22荷瘤小鼠 免疫功能 抗氧化 |
| 英文关键词: Total anthraquinone from Zhushaqi H22 tumor bearing mice Immune function Antioxidation |
| 基金项目:陕西省自然科学基础研究计划项目（2017JM8021） |
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| 中文摘要: |
| 摘要 目的：探讨不同剂量朱砂七总蒽醌对H22荷瘤小鼠免疫功能及抗氧化能力的影响。方法：选取清洁级昆明小鼠60只，按照随机数字表法分为正常对照组、H22荷瘤组、环磷酰胺组、低剂量组、中剂量组、高剂量组，每组各10只。除正常对照组外，其余5组小鼠建立H22荷瘤小鼠模型。低剂量组、中剂量组、高剂量组分别给予0.3 g/kg、0.6 g/kg、1.2 g/kg的朱砂七总蒽醌悬浊液干预，环磷酰胺组给予0.02 g/kg的环磷酰胺干预，正常对照组和H22荷瘤组给予等剂量的1%的羧甲基纤维素钠干预。比较各组小鼠的肿瘤体质量、抑瘤率、T淋巴细胞亚群以及血清超氧化歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）、乳酸脱氢酶（LDH）水平。结果：环磷酰胺组、高剂量组的肿瘤体质量低于低剂量组，抑瘤率高于低剂量组，差异具有统计学意义（P<0.05）。高剂量组的CD4+、CD4+/CD8+均高于环磷酰胺组、低剂量组、中剂量组，CD8YT1118883439292低于环磷酰胺组、低剂量组、中剂量组，差异均有统计学意义（P<0.05）。高剂量组血清SOD、GSH-Px水平高于其他5组，MDA、LDH水平低于H22荷瘤组、低剂量组、中剂量组，差异均有统计学意义（P<0.05）。结论：朱砂七总蒽醌具有明显的抗肿瘤作用，可增强小鼠的免疫功能和抗氧化能力，且具有剂量效应。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of different doses of total anthraquinone from Zhushaqi on immune function and antioxidant capacity in H22 tumor bearing mice. Methods: 60 clean grade Kunming mice were selected, and the mice were divided into normal control group, H22 tumor bearing group, cyclophosphamide group, low dose group, medium dose group and high dose group ac- cording to random number table method, with 10 mice in each group. In addition to the normal control group, the mice in other 5 groups were established H22 tumor bearing mice model. Low dose group, middle dose group and high dose group were given 0.3 g/kg, 0.6 g/kg,1.2 g/kg total anthraquinone from Zhushaqi suspension intervention, respectively, cyclophosphamide group was given 0.02 g/kg cy- clophosphamide intervention, normal control group and H22 tumor bearing group were given equal dose of 1% sodium carboxymethyl cellulose intervention. Tumor mass, tumor inhibition rate, T lymphocyte subsets, levels of serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and lactate dehydrogenase (LDH) were compared between the mice in each group. Results: The tumor mass in cyclophosphamide group and high-dose group was lower than that in low dose group, the tumor inhibition rate was higher than that of low dose group, and the difference was statistically significant (P<0.05). The CD4+ and CD4+/CD8+ of high dose group were higher than those of cyclophosphamide group, low dose group and middle dose group, and CD8+ was lower than that of cyclophosphamide group, low dose group and middle dose group, the difference was statistically significant (P<0.05). The levels of serum SOD and GSH-Px in the high dose group were higher than those in the other 5 groups, and the levels of MDA and LDH were lower than those in the H22 tumor bearing group, low dose group and medium dose group (P<0.05). Conclusion: Total Anthraquinone from Zhushaqi has obvious anti-tumor effect, it can enhance the immune function and antioxidant capacity of mice, and it has dose effect. |
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