| 蒋丽霞,徐家柱,杨小元,魏长征.快速膨胀片层多孔壳聚糖止血海绵的制备及生物相容性研究[J].现代生物医学进展英文版,2018,(15):2829-2835. |
| 快速膨胀片层多孔壳聚糖止血海绵的制备及生物相容性研究 |
| Preparation of Rapid Swelling Chitosan Hemostatic Sponges with Lamellar Porous and Their Biocompatibility Evaluation |
| Received:November 30, 2017 Revised:December 26, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.15.006 |
| 中文关键词: 快速膨胀 片层多孔 壳聚糖止血海绵 |
| 英文关键词: Rapid swelling Lamellar porous Chitosan hemostatic sponges |
| 基金项目:国家高技术研究发展计划（国家863）项目（2014AA09） |
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| 中文摘要: |
| 摘要 目的：探索快速膨胀片层多孔壳聚糖止血海绵的制备工艺，评价止血海绵的理化性能及生物相容性，并探讨原料脱乙酰度对止血海绵性能的影响。方法：考察止血海绵的理化性质，包括扫描电子显微镜（SEM）观察表观形貌，检测力学性能、吸水率、快速吸水膨胀时间和膨胀率，研究其体内外的生物相容性，包括体外细胞毒性实验、动物皮内刺激实验和皮下植入实验。结果：确定了止血海绵的制备工艺，采用该工艺制备的止血海绵均具有片层多孔结构，且具有较高的力学强度和快速膨胀的特点。证实高脱乙酰度原料（DD=95.14 %）制备的止血海绵力学性能、吸水率、膨胀率均优于低脱乙酰度原料（DD= 69.70 %）制备的止血海绵。脱乙酰度69.70 %和脱乙酰度95.14 %的壳聚糖止血海绵，拉伸强度分别为10.1 N和15.4 N，吸水率分别为1904 %和2131 %，吸水膨胀时间分别为13.4 s和14.0 s，膨胀率分别为8.4倍和10.8倍。体外细胞毒性实验表明脱乙酰度为95.14 %的壳聚糖止血海绵更有利于细胞的增殖，皮内刺激和皮下植入实验结果表明脱乙酰度为95.14 %的壳聚糖海止血海绵表现出更小的组织炎性反应。结论：脱乙酰度为95.14 %的壳聚糖止血海绵具有优良的力学性能、优异的吸水膨胀能力以及良好的生物相容性，在临床止血特别是腔隙止血方面具有广阔的应用前景。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the technique of preparing rapid swelling chitosan hemostatic sponges with lamellar porous structure, evaluate their physical and chemical properties as well as biocompatibility, and investigate the influence of deacetylated degree (DD) of raw chitosan on hemostatic properties. Methods: The morphology of chitosan hemostatic sponges were observed using SEM, the mechanical properties, water absorption rate, water swelling time as well as the swelling ratio were examined. The biocompatibilities of chitosan hemostatic sponges were evaluated both in vitro and in vivo, including cytotoxicity test, intracutaneous reactivity test and subcu- taneous implant. Results: With the determined fabrication technique, chitosan hemostatic sponges with different DD both exhibited lamellar porous structure and high mechanical strength as well as the property of rapid swelling. The mechanical strength, water absorp- tion rate and swelling ratio of chitosan hemostatic sponges with high DD (95.14 %) were higher than that of chitosan with low DD (69.70 %). The mechanical strength, water absorption rate, swelling time and swelling ratio of hemostatic sponge fabricated from chitosan with low (69.70 %) and high (95.14 %) DD were 10.1 N and 15.4 N, 1904 % and 2131 %, 13.4 s and 14.0 s, 8.4 times and 10.8 times, respec- tively. In vitro cytocompatibility test indicated that the chitosan hemostatic sponges significantly promoted the proliferation of cells. In vi- vo intracutaneous reactivity test and subcutaneous implant test demonstrated that the chitosan hemostatic sponge with high DD (95.14 %) had excellent biocompatibility and less inflammatory response. Conclusion: Chitosan hemostatic sponges with high DD (95.14 %) ex- hibited better mechanical strength, water absorption rate, swelling rate and biocompatibility and thus demonstrated their potential to be used in hemostatic application. |
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