| 韩跃东,张 超,张 松,张衍国.蝇蛆油促进大鼠皮肤机械损伤愈合作用及机制研究[J].现代生物医学进展英文版,2018,(14):2641-2646. |
| 蝇蛆油促进大鼠皮肤机械损伤愈合作用及机制研究 |
| Effects of Maggot Oil on the Healing of Rat Skin Mechanical Damage and Its Possible Mechanism |
| Received:September 23, 2017 Revised:October 18, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.14.008 |
| 中文关键词: 蝇蛆油 皮肤机械损伤 愈合 |
| 英文关键词: Maggot Oil Skin mechanical damage Wound healing |
| 基金项目:陕西省重点研发计划项目（2017SF-136） |
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| 中文摘要: |
| 摘要 目的：本文拟研究蝇蛆油对皮肤机械损伤的治疗作用及作用机制。方法：SD大鼠随机分为正常组、模型组、蝇蛆油组和重组牛碱性成纤维细胞生长因子（rb-bFGF）凝胶组，制作大鼠皮肤机械性损伤模型，在不同时间点采集样本，检测创面愈合率、创面愈合时间、血管内皮生长因子（VEGF）含量、丙二醛（MDA）和超氧化物歧化酶（SOD）水平，并检测组织中胶原蛋白I、胶原蛋白III、血小板衍生因子（PDGF）和细胞角蛋白19（CK19）表达情况。结果：模型组大鼠创面愈合时间为29.5±2.6 d，而蝇蛆油创面愈合时间为22.4±2.8 d，存在显著差异（P<0.01）。VEDF含量在模型组和给药组均随着创面愈合时间增加，给药组在第1 d即显著高于模型组（P<0.01），并在第21 d达到最大值（2051.5±148.2 ng/L）。模型组大鼠创面组织中MDA含量为6.47±0.92 nmoL/mg，SOD含量为7.52±3.21 U/mg，而蝇蛆油给药组大鼠创面组织中MDA含量为3.42±0.83 nmoL/mg，SOD含量为21.32±2.94 U/mg，存在显著差异（P<0.01）。进一步研究发现，与模型组比较，蝇蛆油给药组大鼠创面组织中胶原蛋白I、胶原蛋白III、PDGF和CK19含量均显著升高（P<0.01）。结论：蝇蛆油能够促进机械性损伤皮肤创面愈合，其作用机制可能是通过促进血管生成，抗氧化损伤，促进胶原生成，诱导干细胞形成，从而促进创面愈合。 |
| 英文摘要: |
| ABSTRACT Objective: In this study, we tried to study the protective effects of Maggot Oil on skin mechanical damage and its pos- sible mechanism in this study. Methods: SD rats were randomly divided into four groups: control group, model group, Maggot Oil treat- ment group and rb-bFGF gel treatment group. The model of skin mechanical damage was built. Samples were collected at different time points. Wound healing rates, wound healing time, VEDF contents, MDA and SOD levels were measured. The expression levels of colla- gen type I, collagen type Ⅲ, PDGF and CK19 in tissues were measured. Results: The healing time of the wound was 29.5±2.6 d in the model group, and the healing time of maggot oil treatment group was 22.4±2.8 d, and there was significant difference between these groups (P<0.01). The contents of VEGF were increased in model and maggot oil treatment group following the wound healing. In maggot oil treatment group, it was significantly higher than model group from the first day (P<0.01), and reached the maximum (2051.5±148.2 ng/L) on the 21st day. The tissue content of MDA was 6.47±0.92 nmol/mg and the tissue content of SOD was 7.52±3.21 U/mg in mod- el group, however, the content of MDA was 3.42±0.83 nmol/mg, and the content of SOD was 21.32±2.94 U/mg in maggot oil treat- ment group, there was significant difference between these groups (P<0.01). Further study showed that the levels of collagen I, colla- gen III, PDGF and CK19 were significantly increased in the maggot oil treatment group compared with the model group (P<0.01). Conclusion: Maggot Oil could protect tissue injury induced by skin mechanical damage, promote wound repair. The possible mechanism might through promoting angiogenesis, anti-oxidative damage, promoting collagen formation, inducing stem cell formation, thus promote wound healing. |
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