| 马志勇,李雪平,侯振宇,汪明强,吕金利.MiRNA-155通过调控β-catenin促进结肠癌SW480细胞的侵袭[J].现代生物医学进展英文版,2018,(12):2242-2247. |
| MiRNA-155通过调控β-catenin促进结肠癌SW480细胞的侵袭 |
| MicroRNA-155 Promotes the Invasion of Colon Cancer Cell SW480 by Upregulating Wnt/β-catenin Signaling Pathway |
| Received:September 17, 2017 Revised:October 12, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.12.009 |
| 中文关键词: 结肠癌 miR-155 β-连环蛋白 侵袭 |
| 英文关键词: Colon cancer Micro RNA-155 Wnt/β-catenin Invasion |
| 基金项目:国家自然科学基金项目（30972894） |
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| 中文摘要: |
| 摘要 目的：探讨microRNA-155(miR-155)对结肠癌细胞SW480侵袭能力的影响及其可能机制。方法：采用反转录－聚合酶链反应(RT-PCR)测定结肠癌组织与邻近正常结肠组织中miR-155的表达。将miR-155 mimic和β-catenin特异性的siRNA(β-catenin siRNA)分别通过脂质体转染法转染入结肠癌SW480细胞，应用RT-PCR检测细胞中miR-155和β-catenin mRNA的表达，采用蛋白质印迹法(Western Blot)检测β-catenin蛋白表达，采用Transwell侵袭实验检测miR-155 mimic及β-catenin siRNA对SW480细胞侵袭能力的影响。结果：结肠癌组织中的miR-155的表达较邻近正常结肠组织明显升高(P＜0.05)；miR-155 mimic可使β-catenin的mRNA和蛋白表达均显著升高(P＜0.05)，同时可显著增强SW480细胞的侵袭能力(P＜0.05)，而转染miR-155 mimic和β-catenin siRNA的SW480细胞侵袭能力较仅转染miR-155 mimic的SW480细胞显著减弱(P＜0.05)。结论：结肠癌组织中miR-155的表达上调，可能通过激活B-catenin信号通路促进肿瘤细胞的远处侵袭转移。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the role of microRNA-155 (miR-155) in the invasion potential of colon cancer cell and the exact underlying mechanism. Methods: The expression level of miR-155 in colon cancer and adjacent normal tissues was detected by real- time quantitative PCR(RT-PCR). miR-155 mimics (miR-155), or siRNA against β-catenin (β-catenin siRNA) were transfected into hu- man colon cancer cell line SW-480 respectively using lipofectamine 2000, and RT-PCR was used to measure the mRNA expression levels of miR-155 and β-catenin, and β-catenin protein expression level was detected by Western blot. The in-vitro cell invasion ability was de- termined by Transwell invasion assays after up-regulating miR-155 or knocking down of β-catenin. Results: The expression levels of miR-155 was higher in colon cancer tissue compared to adjacent normal tissues. miR-155 directly up-regulates the mRNA and protein expression levels of β-catenin. In addition, miR-155 enhances cell invasion abilities, whereas the invasion potentiality was decreased af- ter co-treated with β-catenin siRNA. Conclusion: miR-155 promotes the invasion potential of colon cancer cell, at least partly through the upregulation of β-catenin. The findings of this study suggest that miR-155 and β-catenin may have a unique potential as a novel biomarker candidate for diagnosis and treatment in tumor metastasis. |
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