| 王晓慧,徐孝娜,张 磊,王 枫,杨瑞华,秦绪军.CIC对高脂膳食大鼠肝脏氧化应激的影响[J].现代生物医学进展英文版,2018,(11):2072-2076. |
| CIC对高脂膳食大鼠肝脏氧化应激的影响 |
| Effect of CIC on Hepatic Oxidative Stress in High-fat Diet Rats |
| Received:November 21, 2017 Revised:December 30, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.11.015 |
| 中文关键词: 冷诱导RNA结合蛋白 冷适应 高脂膳食 氧化应激 |
| 英文关键词: Cold inducible RNA binding protein Cold adaptation High-fat diet Oxidative stress |
| 基金项目:陕西省自然科学基金面上项目（2016JM8048）；国家自然科学基金面上项目（31670863） |
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| 中文摘要: |
| 摘要 目的：研究慢性间断性冷暴露（mild chronic intermittent cold exposure，CIC）对高脂膳食大鼠肝脏氧化应激的影响。方法：轻度CIC已被广泛用于建立冷适应研究的动物模型。本研究通过将大鼠暴露于温和的CIC和/或高脂膳食4w，检测肛温、体重、肝脏重量、ATP和活性氧（ROS）的水平，Western blot检测冷诱导RNA结合蛋白（cold inducible RNA binding protein，Cirbp）和硫氧还蛋白（Thioredoxin，TRX）的蛋白表达。结果：同对照组相比，高脂膳食组体重显著增加，血清和肝脏ROS水平显著升高，ATP水平没有显著影响。同对照组相比，CIC暴露1w后大鼠肛温显著降低，而2w、3w和4w周肛温没有显著差异，ROS水平无显著差异，但ATP水平显著升高；Cirbp和TRX的表达显著升高。同常温高脂膳食组相比，CIC暴露4w后，大鼠体重显著降低，ROS水平无显著差异，而ATP水平显著升高；Cirbp和TRX的表达水平显著升高。这些结果均提示冷适应增强了高脂膳食大鼠肝脏的抗氧化水平，可能是由于冷适应后Cirbp表达升高，继而调控其下游的抗氧化蛋白TRX的表达增加，从而清除ROS的缘故。结论：CIC暴露诱导的冷适应可保护肝脏免于高脂膳食诱导的氧化应激。 |
| 英文摘要: |
| ABSTRACT Objective: The main aim of this study is to investigate the beneficial effects of mild chronic intermittent cold (CIC) exposure on the effects of high-fat diet feeding (HFD) on liver oxidative stress in SD rats. Methods: Mild CIC has been widely used to establish the animal model of cold adaptation. HFD (45% of fat) and CIC (4℃, 6 hours/day) were given concurrently to the rats for 4 weeks. The rectal temperature, body weight, liver weight, the levels of ATP and reactive oxygen species (ROS) in liver are measured after 4 weeks of rats exposed to mild CIC and / or HFD. The protein level of cold inducible RNA binding protein (Cirbp) and thioredoxin (TRX) are detected by western blotting. Results: Compared with the control group, the body weight of the high fat diet group increases significantly, the ROS level increases significantly, and the level of ATP has no significant effect; the rectal temperature of rats decreases significantly after 1 d and 1 w of CIC exposure, while the rectal temperature has no significant difference after CIC exposure of 2 w, 3 w and 4 w; there's no significant difference in the level of ROS, but the ATP level and the expression of Cirbp and TRX are increased significantly. Compared with HFD group under normal temperature, the body weight decreases significantly after CIC exposure 4 w, the level of ROS had no significant difference, while ATP level increased significantly; the expression of Cirbp and TRX increased significantly. These results suggest that cold adaptation enhances the antioxidant level in the liver of HFD rats, probably due to the increased expression of Cirbp after cold acclimation, and then increases the expression of the antioxidant protein TRX, thus cleaning ROS. Conclusion: Cold adaptation induced by CIC exposure protects the liver against oxidative stress induced byHFD. |
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