| 樊姝彤,满 怡,梁祖穆,李清华,陈丽梅,郭璐瑶,潘智芳,冯卫国.ZNF185基因抗原表位分析及合成肽免疫避孕效果研究[J].现代生物医学进展英文版,2018,(11):2052-2056. |
| ZNF185基因抗原表位分析及合成肽免疫避孕效果研究 |
| Antigenic Epitopes Analysis of ZNF185 Gene and Study on the Effect of Synthetic Peptide Immunocontraception |
| Received:December 20, 2017 Revised:January 26, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.11.011 |
| 中文关键词: ZNF185 抗原表位 合成肽 免疫避孕 |
| 英文关键词: ZNF185 Antigenic epitopes Synthetic peptides Immune contraception |
| 基金项目:山东省科技发展计划项目(2015GSF118178)；山东省卫计委科技项目(201411)；山东省自然科学基金项目（ZR2015HL021） |
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| 中文摘要: |
| 摘要 目的：预测ZNF185基因的抗原表位，并研究相应合成多肽诱导的免疫应答和免疫避孕效应。方法：应用DNA Star软件预测ZNF185蛋白二级结构、亲水性、可塑性、表面可及性与抗原指数。合成携带ZNF185抗原表位的多肽，进行小鼠免疫实验，检测小鼠血清中IgG抗体水平、交配率、妊娠率及每窝产仔数，并观察生殖器官是否发生病变。结果：筛选ZNF185两段氨基酸序列合成多肽，该多肽能够诱导小鼠产生较高的抗体水平，实验组小鼠交配率和妊娠率显著低于对照组，生殖器官并无发生明显的组织病理学变化。结论：ZNF185具有一定的抗生育作用。 |
| 英文摘要: |
| ABSTRACT Objective: To predict the antigenic epitopes of the ZNF185 gene and investigate the immune response and immune contraceptive effect induced by corresponding synthetic peptides. Methods: The secondary structure, hydrophilicity, plasticity, surface probability and antigen index of ZNF185 protein were predicted by DNA S tar software. The synthetic polypeptide carrying antigenic epitopesof ZNF185was used to vaccinate mice, and thenthe IgG antibody levels in mouse serum, mating rate, fertility rate and mean litter size were examined. In addition, pathologic change of the reproductive organs was observed. Results: Two amino acid sequences of ZNF185 were screened to synthesize polypeptide, the polypeptide could induce mice to produce higher antibody levels. Furthermore, the rate of mating and fertility was significantly lower in the experimental group than in the control group, and no show significant histopathological changes was observed in reproductive organs. Conclusion: ZNF185 has a certain anti-fertility effect. |
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