| 党引利,徐 溪,陈丽展,张 瑶,白 丹,吴 朔.干扰素IFNL4对巨噬细胞系THP-1免疫应答的影响[J].现代生物医学进展英文版,2018,(11):2047-2051. |
| 干扰素IFNL4对巨噬细胞系THP-1免疫应答的影响 |
| The Effects of IFNL4 Expression on Immune Response of Macrophages in THP-1 Cell System |
| Received:November 21, 2017 Revised:December 15, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.11.010 |
| 中文关键词: IFNL4 THP-1巨噬细胞 免疫应答 细胞因子 巨噬细胞迁移 |
| 英文关键词: IFNL4 THP-1 macrophage Immune response Cytokines Macrophage migration |
| 基金项目:国家自然科学基金项目（81601553） |
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| 中文摘要: |
| 摘要 目的：分析Interferon-lambda 4（IFNL4）表达与巨噬细胞免疫应答间的关系，探讨IFNL4调控免疫应答的信号机制，发掘IFNL4在免疫调理方面的潜在应用价值。方法：建立THP-1细胞培养及分化刺激体系，使用RT-PCR检测不同分化状态THP-1细胞IFNL4的表达水平，并在THP-1细胞中过表达IFNL4，检测IFNL4过表达对THP-1细胞分泌IL-12、TNF-α、IL-10和TGF-β等细胞因子及细胞迁移效率的影响。结果：THP-1细胞分化抑制IFNL4的表达，分化前比分化后IFNL4表达水平相对定量下降255.46倍，差异显著性P<0.001；M2极化巨噬细胞较M1细胞表达IFNL4因子水平升高14.69倍，显著性差异P=0.009；IFNL4过表达可抑制IL-12和TNF-α表达水平，其中TNF-α表达水平变化具有统计学意义（P=0.017），表达水下降5.97倍。IFNL4可促进IL-10和TGF-β的表达，其中TGF-β变化具有统计学意义（P=0.046），表达水平相对上升2.42倍。且IFNL4对THP-1细胞迁移效率具有抑制作用（P=0.005），刺激前细胞迁移数为45.33，IFNL4刺激后迁移数为32.67，迁移移效率下降1.39倍。结论：干扰素IFNL4在分化的M2型THP-1巨噬细胞中具有较高的表达水平，且对THP-1的免疫应答具有一定的抑制作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between IFNL4 expression and macrophage functions, analyze the role of IFNL4 in immune regulation, and explore for the potential application value of IFNL4 in immune regulation. Methods: The THP-1 cell culture and stimulation system was esteblished. The IFNL4 expressions in different type of THP-1 cells were detected by RT-PCR. IFNL4 was then overexpressed in THP-1 cells, and the expression of IL-12, TNF-α, IL-10 and TGF-β in these cells were analyzed by RT-PCR. The migration efficiency of THP-1 cells was analyzed by transwell assay before or after IFNL4 overexpression. Results: The differentiation of THP-1 cells inhibited IFNL4 expression (P<0.001), and M2 macrophage can secrete more IFNL4 comparing to M1 macrophage (P=0.009). IFNL4 overexpression in THP-1 cells inhibit IL-12 and TNF-α secretion while promote IL-10 and TGF-β expression, in which TNF-α and TGF-β variations were significantly different (P=0.017 and p=0.046, respectively). Furthermore, IFNL4 overexpression inhibit THP-1 cell migration significantly (P=0.005). Conclusion: IFNL4 has a certain inhibitory effect on inflammation function of macrophage in THP-1 cell system. |
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