| 熊春雷,姚青林,冯 品,王晓光,张静宜.低频交变磁场刺激对缺铁性贫血大鼠贫血改善的作用研究[J].现代生物医学进展英文版,2018,(11):2038-2042. |
| 低频交变磁场刺激对缺铁性贫血大鼠贫血改善的作用研究 |
| The Mitigatory Effects of Low-frequency Alternating Electromagnetic Fields on Iron-deficiency Anemia in Rats |
| Received:November 11, 2017 Revised:December 22, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.11.008 |
| 中文关键词: 交变电磁场 缺铁性贫血 大鼠 血红蛋白 血清铁 |
| 英文关键词: Alternating electromagnetic fields Iron-deficiency anemia Rat Hemoglobin Serum iron |
| 基金项目:国家自然科学基金项目（81301075） |
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| 中文摘要: |
| 摘要 目的：明确低频正弦波交变电磁场对缺铁性贫血大鼠贫血改善的作用效果，为其未来的临床应用提供实验依据。方法：雄性断乳的SPF级Sprague-Dawley（SD）大鼠，共36只，随机的等分为空白对照组（n=12）、缺铁性贫血组（n=12）和缺铁性贫血+电磁场刺激组（n=12）。缺铁性贫血组和缺铁性贫血+电磁场刺激组的大鼠饲养以低铁饲料和去离子水，每周尾静脉放血1 mL。空白对照组大鼠饲养以常规饲料和普通蒸馏水，且不予尾静脉放血。对缺铁性贫血+电磁场刺激组的12只大鼠施加全身低频交变电磁场刺激，每天刺激2小时，连续刺激10周。实验结束后提取大鼠血液样本，使用氰化高铁血红蛋白法进行测定全血血红蛋白含量，使用专用试剂盒测定血清铁和总铁结合力；提取肝脏和脾脏组织，对肝脏铁和脾脏铁含量进行测定。结果：全身暴露低频交变电磁场刺激显著提高了缺铁性贫血大鼠体重（P<0.05），提升了其血清铁含量（P<0.05），显著提高全血血红蛋白含量（P<0.05），并显著降低了缺铁性贫血大鼠血清总铁结合力（P<0.05）；同时，电磁刺激也显著提高了缺铁性贫血大鼠肝脏铁和脾脏铁含量（P<0.05）。结论：交变电磁场作为一种经济、安全、无创的物理作用方式，具有较为显著的缺铁性贫血的改善效果。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the potential effects of low-frequency sin-wave alternating electromagnetic fields on iron-deficiency anemia in rats, and to offer experimental evidence for its future potential clinical application. Methods: Thirty six weaning male Sprague-Dawley (SD) rats were randomly and equally assigned into the blank control (Control, n=12), iron-deficiency anemia (IDA, n=12) and iron-deficiency anemia with alternating electromagnetic fields stimulation (IDA+EMF, n=12) groups. The rats in the IDA and IDA+EMF groups were fed with low-iron diet and deionized water, and also subjected to blooding at caudal vein once per week. The rats in the blank control group were fed with the regular rodent chow and distilled water without blooding at caudal vein. The rats in the IDA+EMF group were exposed to whole-body sin-wave alternating electromagnetic magnetic fields with 2 hours per day for 10 consecutive weeks. After the experiment, all rats were sacrificed and the blood samples were collected. The whole-blood hemoglobin concentration was determined using the cyanomethemoglobin method, and the serum iron and total iron binding capacity were quantified using the specific kits. The liver and spleen tissues were also extracted and the liver iron and spleen iron levels were also determined. Results: Whole-body exposure to alternating electromagnetic fields significantly increased the body weights in iron-deficiency anemia rats (P<0.05), enhanced serum iron secretion (P<0.05), promoted whole-blood hemoglobin expression (P<0.05), and also decreased total iron binding capacity in iron-deficiency anemia rats (P<0.05). Moreover, electromagnetic stimulation also significantly increased the liver iron and spleen iron concentrations in iron-deficiency anemia rats (P<0.05). Conclusion: Treatment with alternating electromagnetic fields, as a kind of economic, safe and non-invasive biophysical method, exhibits significantly ameliorative effects on iron-deficiency anemia. |
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