| 初 旭,刘永兴,党新文,周永红,赵 咪.microRNA-21对人舌鳞癌细胞的增殖和凋亡的影响[J].现代生物医学进展英文版,2018,(10):1940-1943. |
| microRNA-21对人舌鳞癌细胞的增殖和凋亡的影响 |
| Effect of microRNA-21 on Proliferation and Apoptosis of Human Tongue Squamous Carcinoma Cell |
| Received:January 20, 2018 Revised:February 15, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.10.027 |
| 中文关键词: miR-21 舌鳞癌 流式细胞术 实时定量荧光聚合酶链式反应 |
| 英文关键词: miR-21 Squamous cell carcinoma of the tongue Flow cytometry real-time quantitative fluorescence polymerase chain reaction |
| 基金项目:国家自然科学基金项目(NSFC81700904) |
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| 中文摘要: |
| 摘要 目的：探讨microRNA-21(miR-21)对人舌鳞癌细胞增殖和凋亡的影响。方法：选取8例舌鳞癌组织和4例癌旁组织为研究材料，采用实时荧光定量聚合酶链式反应（qRT-PCR）法对舌鳞癌及癌旁组织中的miR-21相对表达量进行检测，利用人工合成的miR-21mimic对人舌鳞癌Tca8113细胞进行瞬时转染，采用qRT-PCR法对Tca8113细胞中miR-21相对表达量进行检测，采用四唑盐比色法（MTT）法对Tca8113细胞增殖情况进行检测，采用流式细胞术对Tca8113细胞周期与凋亡情况进行检测。结果：舌鳞癌组织中miR-21的相对表达量(3.502±0.674)，高于癌旁组织(0.998±0.192)，差异有统计学意义(P<0.05)。miR-21mimic导致了Tca8113细胞中的miR-21相对表达量上调(6.864±1.324)，明显高于对照scramble 组[(0.997±0.187), P<0.05]，对Tca8113细胞的增殖发挥了促进作用(P<0.05)。经miR-21mimic转染之后，Tca8113细胞进入S期的细胞出现了明显的增加[( 27.4±5.1)% vs (48.6±8.7)%, P<0.05]，处于G1期的细胞出现了显著的减少[(56.3±9.6)% vs (36.2±7.2)%, P<0.05]，细胞凋亡数量出现了显著减少[(9.4±2.3)% vs (18.6±3.9)%, P<0.05]。结论：miR-21在舌鳞癌组织中高表达，过表达miR-21有效促进了Tca8113细胞的增殖，抑制细胞的凋亡，miR-21在舌鳞癌诊断和治疗可能具有一定的新型靶点价值。 |
| 英文摘要: |
| ABSTRACT Objective: The aim of this study is to investigate the effect of microRNA-21(miR-21) on proliferation and apoptosis of human tongue squamous carcinoma cell. Methods: The miR-21 expression was detected in 8 tongue squamous cell carcinoma tissues and 4 adjacent tissues by Real-time quantitative PCR (qRT-PCR). Artificially synthesized miR-21 mimic was transiently transfected into hu- man tongue squamous cell carcinoma Tca8113 cell, and then the expression of miR-21 was tested by qRT-PCR. MTT assay was used to detect the cell proliferation of Tca8113 cell, apoptosis and cell cycle of Tca8113 cell were measured by flow cytometr. Results: The miR-21 expression of tongue squamous carcinoma tissues was higher than that in adjacent tissues [(3.502±0.674) vs (0.998±0.192), (P<0.05)]. The transient transfection of miR-21 mimic into Tca8113 cell significantly increased the miR-21expression of Tca8113 cell[(6.864±1.324) vs(0.997±0.187), P<0.05], promoted cell proliferation, raised the cell proportion in S phase[( 27.4±5.1)% vs (48.6±8.7)%, P<0.05], reduced the cell proportion in G1 phase [(56.3±9.6)% vs (36.2±7.2)%, P<0.05], and inhibited cell apoptosis[(9.4±2.3)% vs (18.6±3.9)%, P<0.05]. Conclusion: miR-21 was over-expressed in tongue squamous carcinoma tissues. Transient transfections of miR-21 mimic lead to increased proliferation and reduced apoptosis in Tca8113 cell, which suggested that miR-21 maybe a target for the treatment of tongue squamous carcinoma. |
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