| 李 楠,刘永明,韩赛平,王新利,董 川,刘 涛,文艳华,刘云燕,刘 宇,陈孝强,马保安.CSE1L在绝经后女性骨关节炎滑膜细胞中的作用探究[J].现代生物医学进展英文版,2018,(10):1865-1869. |
| CSE1L在绝经后女性骨关节炎滑膜细胞中的作用探究 |
| A Study with Regard to How CSE1L Works in Osteoarthritis among Postmenopausal Women |
| Received:December 26, 2017 Revised:January 21, 2018 |
| DOI：10.13241/j.cnki.pmb.2018.10.012 |
| 中文关键词: CSE1L 绝经 骨性关节炎 滑膜细胞 TNF-α |
| 英文关键词: CSE1L Pausimenia Osteoarthritis Synoviocyte TNF-α |
| 基金项目:国家自然科学基金项目(81372297) |
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| 中文摘要: |
| 摘要 目的：探讨CSE1L在绝经后女性骨关节炎滑膜细胞中的表达及其作用和机制。方法：通过RT-PCR、Western blot以及免疫组化的实验方法检测和比较CSE1L在绝经后女性骨关节炎和创伤截肢患者关节滑膜细胞中的表达，不同浓度TNF-α或TNF-α抗体处理的关节炎滑膜细胞中CSE1L的表达；采用siRNA干预其骨关节炎滑膜细胞中CSE1L的表达后，通过RT-PCR、ELISA以及Western blot检测其TNF-α的表达和分泌情况。结果：RT-PCR以及Western blot结果均提示绝经后女性骨关节炎滑膜细胞中CSE1L的表达较创伤截肢患者关节滑膜细胞显著增高(P<0.01，P< 0.001)。对CSE1L进行siRNA干预降低其表达水平后，CSE1L表现出与TNF-α浓度呈正相关的变化趋势；此外，在对CSE1L进行敲减以降低其表达水平后，绝经后女性骨关节炎滑膜细胞中TNF-α的表达水平变化提示其受到CSE1L调节。结论：CSE1L可能通过调节TNF-α的表达促进绝经后女性骨关节炎的发展。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate whether the expression of CSE1L elevates in the synoviocytes among postmenopausal women with osteoarthritis(OA), after the positive result came out, to futher preliminary look into the mechanism of how CSE1L works in the syn- oviocytes among postmenopausal women with OA. Methods: Investigate whether the expression of CSE1L elevates in the synoviocytes among postmenopausal women with OA by means of RT-PCR, western blot and immunohistochemistry, and then conduct quantitative and semi-quantitative analysis. After knocking down the expression of CSE1L with CSE1L specific siRNA, to investigate whether CSE1L shows a positive relationship with the severity of OA or not by means of western blot. Through the methods of RT-PCR，ELISA and western blot, the interaction between CSE1L and TNF-α was preliminarily illustrated, so the mechanism of how CSE1L works in the synoviocytes among postmenopausal women with OA was preliminarily showed up. Results: In the synociocytes among post- menopausal women with OA, the results of both RT-PCR and western blot manifested that the expression of CSE1L elevated significantly(P<0.01, P<0.001). Moreover, after knocking down the expression of CSE1L with CSE1L specific siRNA, the expression level of CSE1L show a positive relationship with TNF-α concentration. In addition, after knocking down the expression of CSE1L with CSE1L specific siRNA, and the expression level of TNF-α came up to be regulated by CSE1L in the synoviocytes among postmenopausal women with OA. Conclusion: CSE1L promote OA development via interaction with TNF-α expressed by the synoviocytes among postmenopausal women with OA. |
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