| 支伟伟,陈丽娜,李 凯,雷 鸣,梁宏亮.MFN1介导的线粒体融合在心肌细胞凋亡中的作用研究[J].现代生物医学进展英文版,2018,(9):1654-1657. |
| MFN1介导的线粒体融合在心肌细胞凋亡中的作用研究 |
| Effect of MFN1 Mediated Mitochondrial Fusion on the Apoptosis of H9C2 Cardiomyocytes |
| Received:November 14, 2017 Revised:December 10, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.09.011 |
| 中文关键词: MFN1 线粒体融合 心肌细胞 凋亡 |
| 英文关键词: MFN1 Mitochondrial fusion Cardiomyocytes Apoptosis |
| 基金项目:陕西省创新能力支撑计划--青年科技新星项目(2017KJXX-05) |
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| 中文摘要: |
| 摘要 目的：探讨线粒体融合关键蛋白MFN1介导的线粒体融合在调控心肌细胞凋亡中的作用。方法：通过siRNA降低体外培养H9C2心肌细胞中MFN1的表达后，采用Western blot检测线粒体细胞色素c (Cyto c)释放及其下游凋亡效应分子Caspase9与Caspase3活性，流式细胞术检测细胞内活性氧(ROS)的产生情况，流式细胞术检测细胞凋亡的情况。结果：干扰MFN1可显著促进H9C2心肌细胞内细胞色素c由线粒体释放至胞浆，促进Caspase9与Caspase3的激活，增加细胞内活性氧ROS产生并提高细胞凋亡率(均P<0.05)。结论：MFN1介导的线粒体融合可保护心肌细胞凋亡，其机制可能与抑制ROS产生与细胞色素C释放有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the role of MFN1-mediated mitochondrial fusion in the regulation of cardiomyocyte apoptosis. Methods: After knocking down of MFN1 with siRNA in H9C2 cardiomyocytes, cytochrome c release, caspase9 and caspase3 activation, intracellular ROS and cell apoptosis were evaluated by western blotting and flow cytometry. Results: 1). MFN1 significantly promoted the release of cytochrome cfrom the mitochondria to the cytosol, activation of caspase9 and caspase3 ROS production and apoptosis of H9C2 cardiomyocytes (all P<0.05). Conclusion: MFN1-mediated mitochondrial fusion protects cardiomyocyte from apoptosis, which could be partially explained by the inhibition of ROS production and cytochrome c release. |
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