| 陈晓宇,席加喜,刘晓霞,蒙明瑜,叶冬梅.丹参酮II-A对万古霉素所致肾小管上皮细胞KIM-1及TGF-β1表达的影响[J].现代生物医学进展英文版,2018,(9):1637-1641. |
| 丹参酮II-A对万古霉素所致肾小管上皮细胞KIM-1及TGF-β1表达的影响 |
| Effects of Tanshinon on the Expressions of KIM-1, TGF-β1 in Vancomycin-induced HK-2 Cells |
| Received:November 01, 2017 Revised:November 23, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.09.008 |
| 中文关键词: 丹参酮II-A 万古霉素VAN HK-2细胞 肾损伤分子1(KIM-1) 转化生长因子-β(TGF-β) |
| 英文关键词: Tanshinon II-A Vancomycin HK-2 cell KIM-1 TGF-β1 |
| 基金项目:广西医药卫生自筹经费计划课题（Z2016579） |
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| 中文摘要: |
| 摘要 目的：研究丹参酮II-A(TSII-A)对万古霉素(vancomycin，VAN)诱导的人肾近曲小管上皮细胞(HK-2)的存活、氧化应激水平和肾损伤分子1(KIM-1)及转化生长因子-β(TGF-β1)表达的影响。方法：将体外培养的HK-2细胞株接种于6孔培养板，分为空白组、模型组、阳性药物组和TSII-A高、中、低剂量组。加入终浓度为100 μg?L-1万古霉素建立VAN损伤HK-2细胞模型，空白组、模型组加入等体积的生理盐水，阳性药物组加入终浓度为2.5 mg?L-1的氨磷汀溶液，TSII-A各剂量组分别予不同浓度的TSII-A处理48 h。进一步通过MTT法测定细胞存活率；裂解细胞取上清液，紫外分光光度法监测细胞内谷胱甘肽(GSH-PX)含量，黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活性、硫代巴比妥酸(TBA)法测定丙二醛(MDA)含量、硝酸还原酶法测定一氧化氮（NO）活性；ELISA 法测定细胞上清液中KIM-1、TGF-β1的浓度；RT-PCR监测KIM-1、TGF-β1 mRNA表达。结果：与空白组比较，模型组的细胞存活率、细胞悬液中的SOD酶活性和GSH-PX含量明显减低，细胞悬液中MDA含量及NO水平明显升高，上清液中KIM-1的浓度明显升高，KIM-1mRNA的相对表达量明显上调(P<0.05)；TGF-β1的浓度及其mRNA的表达差异无统计学意义(P>0.05)；与模型组比较，阳性组和TSII-A高、中、低剂量组的细胞存活率、细胞悬液中的SOD酶活性和GSH-PX含量明显升高，细胞悬液中MDA含量及NO水平明显降低，上清液中KIM-1的浓度明显降低，KIM-1mRNA的相对表达量明显下调(P<0.05)，且作用均有浓度依赖性。TGFβ1的浓度及其mRNA的表达差异无统计学意义(P>0.05)。结论：本研究结果显示TSII-A以剂量依赖性方式减轻VAN所致HK-2细胞损伤，可能机与其减轻氧化应激有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of tanshinon on the expression of KIM-1, TGF-β1 in vancomycin-induced HK-2 cells. Methods: HK-2 cells were randomly divided into six groups: normal saline(NS) group, model group, positive control group, high dose group, middle dose group and low dose group of TSII-A, The model were duplicated with addition of VAN(100 μg?L-1). The model group, positive control group, high dose group, middle dose group and low dose group of TSII-A were treated by saline solutions, Ami- fostine, panax notoginsenosides(100, 50, 25 mg?L-1). The cell viability was detected with MTT method, the content of MDA, NO and the activity of SOD, GSH-PX were measured and cell structure was observed. The concentration of KIM-1, TGF-β1 were measured by ELISA, the expressions of KIM-1, TGF-β1 mRNA were measured by RT-PCR. Results: Compared with the model group, the cell viabili- ty and activity of SOD, GSH-PX of TSII-A groups and positive control group were significantly increased (P<0.05); the content of MDA, NO, KIM-1 were significantly decreased(P<0.05); the expression of KIM-1 mRNA were significantly decreased(P<0.05); the cell struc- ture was significantly improved, there was no statistical difference(P>0.05) in the concentration of TGF-β1 and the expression of TGF-β1 mRNA. Conclusion: TSII-A can promote the proliferation of HK-2 cells induced by VAN through relieving the oxidation stress. |
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