| 张倩玉,许 斌,张惠博,陈罗军,宋启斌.SD大鼠急性放射性皮炎模型的建立[J].现代生物医学进展英文版,2018,(8):1419-1424. |
| SD大鼠急性放射性皮炎模型的建立 |
| Establishment of a SD Rat Model of Acute Radiation-induced Skin Reaction |
| Received:July 31, 2017 Revised:August 23, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.08.004 |
| 中文关键词: 急性放射性皮炎 SD大鼠模型 电子线 X线 |
| 英文关键词: Acute radiation-induced skin reaction SD rat model Electron irradiation X-ray irradiation |
| 基金项目:国家自然科学基金项目（81372407） |
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| 中文摘要: |
| 摘要 目的：建立安全的SD大鼠急性放射性皮炎模型。方法：将28只雄性SD大鼠随机分为7组，分别为空白对照组，电子线照射组（60 Gy，45 Gy，30 Gy），X线照射组（45 Gy，30 Gy，15 Gy），每组4只。选择臀背部皮肤，去毛后照射。放疗后第一天起开始观察动物皮肤表现，采用Douglas and Fowler评分方法记录每只动物皮炎情况，并定期测量动物体重，观察动物一般情况并记录死亡情况。于放疗后第28天处死动物，取照射区域皮肤行HE染色及免疫组化染色（CD3，CD11c，CD68，IV型胶原），以通过光镜分析射线照射后皮肤组织变化情况、真皮层内炎症细胞浸润类型及胶原形成情况。结果：至放疗后第28天X线照射组动物出现大量死亡，电子线照射组动物均存活，电子线各组均出现不同程度的放射性皮炎反应，镜下可见局部组织不同程度的表皮层坏死、炎症细胞浸润、毛囊及附属器减少等表现，以电子线照射60 Gy及45 Gy组表现明显。免疫组化结果显示放射线照射可使真皮层内以CD68为表面标志的巨噬细胞浸润增加，并促进以IV型胶原为标志胶原细胞形成。结论：电子线60 Gy及45 Gy照射SD大鼠臀背部皮肤可建立一种安全有效的急性放射性皮炎动物模型，其临床表现及病理表现可用于实验研究。 |
| 英文摘要: |
| ABSTRACT Objective: To establish a pre-clinical SD rat model of acute radiation-induced dermatitis. Methods: 28 male SD rats were divided into 7 groups randomly, including control group, electron irradiation groups receiving 60 Gy × 1f, 45 Gy × 1f or 30 Gy × 1f respectively, and X-ray irradiation groups receiving 45 Gy × 1f, 30 Gy × 1f or 15 Gy × 1f respectively. Each group composed 4 rats. All animals were irradiated in shaved dorsal areas（3 cm×3 cm）. The cutaneous damage was assessed during 1-28 days after irradiation using Douglas and Fowler scores. Meanwhile, the weight change and survival of rats were recorded. Irradiated skin tissues were collected for H&E staining and IHC examinations (CD3, CD11c, CD68 and Type IV-collagen) to analyze the pathological change of skin structure, infiltration of immune cells, as well as collagen deposition. Results: High mortality occurred in X-ray irradiation groups, while no death in electron irradiation groups. Distinct degrees of radiation-induced dermatitis were observed in electron irradiation groups. H&E staining showed necrosis in epidermis, inflammatory immune cells infiltration, loss of hair follicle and other skin adnexal, especially in 60 Gy × 1f and 45Gy × 1f groups. IHC results suggested radiation promoted both infiltration of macrophages marked with CD68 in dermis and collagen formation marked with type Ⅳ-collagen. Conclusion: Irradiating with 60 Gy and 45 Gy electron on SD rats successfully established a practically pre-clinical animal model. The clinical and pathological features could be employed in future research. |
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