| 顾 婷,徐占玲,曹 玲,雷 霞,刘海洋,耿 放,刘国良,张 宁.圣草酚对Aβ25-35诱导的PC12细胞损伤的保护作用及其机制[J].现代生物医学进展英文版,2018,(7):1230-1233. |
| 圣草酚对Aβ25-35诱导的PC12细胞损伤的保护作用及其机制 |
| Effect of Eriodictyol on the Cholinergic System in Aβ25-35-induced PC12 Cells and Its Mechanism |
| Received:October 16, 2017 Revised:October 31, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.07.006 |
| 中文关键词: 圣草酚 Aβ25-35 PC12细胞 胆碱能系统 |
| 英文关键词: Eriodictyol Aβ25-35 PC12 cells Cholinergic system |
| 基金项目:国家自然科学基金面上项目（81673581）；哈尔滨市科技创新人才研究专项资金（2016RQYXJ012）；黑龙江中医药大学新药研究基金项目（2015xy04) |
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| 中文摘要: |
| 摘要 目的：探讨圣草酚对Aβ25-35诱导的PC12细胞损伤的保护作用及其机制。方法：采用MTT法筛选圣草酚对Aβ25-35诱导的PC12细胞损伤的有效保护浓度，进一步采用流式细胞术检测圣草酚对Aβ25-35诱导的PC12细胞的凋亡率的影响，并通过试剂盒检测圣草酚对Aβ25-35诱导的PC12细胞胆碱能系统受体活性的影响。结果：MTT结果显示2×10-3 μmol/L圣草酚可显著促进Aβ25-35诱导的PC12细胞增殖(P<0.05)。流式细胞术结果显示2×10-3 μmol/L圣草酚和雌二醇均可以显著抑制Aβ25-35诱导的PC12细胞凋亡(P<0.05)，提高其乙酰胆碱及乙酰胆碱转移酶活性并且降低乙酰胆碱酯酶的活性(P<0.05)，且二者的作用相当，差异无统计学意义(P>0.05)。结论：圣草酚可能通过提高Ach及ChAT的含量，降低AchE的含量，调节胆碱能系统相关酶的活性，发挥对Aβ25-35诱导的PC12细胞的保护作用。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the protection effects and it's mechanism of eriodictyol on PC12 cell injury induced by Aβ25-35. Methods: MTT assay was used to choose the effective concentration of eriodictyol to Aβ25-35 injured PC12 cells, furthermore, flow cytom- etry was used to detect the effect of eriodictyol on the apoptosis rate of PC12 cells induced by Aβ25-35. The effect of eriodictyol on the ac- tivity of cholinergic receptor in PC12 cells induced by Aβ25-35 was detected by Kit. Results: MTT results showed that 2×10-3 μmol/L could significantly promote the proliferation of PC12 cells induced by Aβ25-35 (P<0.05). Flow cytometry showed that 2×10-3 μmol/L eriodictyol and estradiol can significantly inhibit the apoptosis of PC12 cell induced by Aβ25-35 (P<0.05), improve the transferase activity and de- creased the activity of acetylcholinesterase and the acetylcholine acetylcholine (P<0.05), and two of us, there was no statistically signifi- cant difference (P>0.05). Conclusion: The eriodictyol can protect the PC12 cells induced by Aβ25-35 through increasing the content of Ach and ChAT, decreasing the content of AchE, regulating the activity of related enzymes in cholinergic system has obvious protective effects on PC12 cell injury induced by Aβ25-35, and its mechanism may be related to the cholinergic system. |
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