| 马玉龙,李 红,周雪琴,王刘倩,王 敏.二甲双胍联合放射线照射对鼻咽癌细胞CNE-1增殖的影响[J].现代生物医学进展英文版,2018,(5):839-842. |
| 二甲双胍联合放射线照射对鼻咽癌细胞CNE-1增殖的影响 |
| Effects of Metformin combined with Radiation Exposure on the Proliferation of Nasopharyngeal Carcinoma Cells CNE-1 |
| Received:November 05, 2017 Revised:November 30, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.05.008 |
| 中文关键词: 二甲双胍 放射线照射 鼻咽癌细胞 细胞增殖 |
| 英文关键词: Metformin Radiation exposure Nasopharyngeal carcinoma cells Proliferation |
| 基金项目:国家自然科学基金项目（30672299） |
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| 中文摘要: |
| 摘要 目的：探讨二甲双胍联合放射线照射对鼻咽癌细胞CNE-1增殖的影响。方法：分别给予鼻咽癌细胞CNE-1二甲双胍(5 mM)、2Gy放射线照射、二甲双胍(5 mM)联合2Gy放射线照射处理后，采用MTT实验、克隆形成实验检测和比较其细胞增殖抑制率和克隆形成抑制率。结果：MTT实验结果显示：与二甲双胍组或2Gy放射线照射组相比，二甲双胍联合放射线照射组细胞增殖抑制率显著升高，差异具有统计学意义(P<0.05)；克隆形成实验结果显示，与二甲双胍组或2Gy放射线照射组相比，二甲双胍联合放射线照射组细胞克隆形成抑制率显著升高，差异具有统计学意义(P<0.05)。结论：二甲双胍联合放射线照射能够有效的抑制鼻咽癌细胞CNE-1的增殖。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of metformin combined with radiation exposure on the proliferation of nasopharyngeal carcinoma cells CNE-1. Methods: CNE-1 cell was treated with metformin (5 mM), 2Gy radiation exposure, metformin (5 mM) combined with 2Gy radiation exposure, respectively. MTT and clone formation assays were used to detect the cell proliferation inhibition rate and clone formation inhibition rate. Results: MTT assay showed that compared with metformin or 2Gy radiation exposure, the cell prolifera- tion inhibition rate of metformin combined with radiation exposure was significantly increased(P<0.05). The clone formation assay showed that compared with metformin or 2Gy radiation exposure, the cell clone formation inhibition rate of metformin combined with ra- diation exposure was significantly increased(P<0.05). Conclusion: Metformin combined with radiation exposure could effectively inhibit the proliferation of nasopharyngeal carcinoma cells CNE-1. |
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