| 吴 琼,杨丹丹,郭菲菲,孙向荣,徐 珞.生长素对胃运动和对海马胃肠敏感神经元放电活动的影响[J].现代生物医学进展英文版,2018,(5):832-838. |
| 生长素对胃运动和对海马胃肠敏感神经元放电活动的影响 |
| The Effects of Ghrelin on Gastric Motility and the Activity of Gastrointestinal Sensitive Neurons in the Hippocampus and the Regulation of Hypothalamic Hormones |
| Received:May 23, 2017 Revised:June 18, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.05.007 |
| 中文关键词: 生长素 下丘脑 胃运动 胃牵张敏感神经元 |
| 英文关键词: Ghrelin Hippocampus Gastric motility Gastrointestinal sensitive neurons |
| 基金项目:国家自然科学基金项目（81470815；81270460；81500414）；山东省优秀中青年科学基金项目（BS2014YY009）；青岛市科技局项目（14-2-3-3-nsh） |
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| 中文摘要: |
| 摘要 目的：探讨海马ghrelin对GD敏感神经元放电和弓状核ghrelin对胃运动的影响。方法：在细胞外记录海马的放电情况，并且检测清醒大鼠的胃运动。通过PCR免疫印迹和免疫荧光组织化学染色等方法来测定GHSR-1a在海马中的表达。用逆行追踪和免疫荧光组织化学染色检测ghrelin神经元的投射情况。Ghrelin况荧光金双标记的神经元以及GHSR-1a的表达分别可以在ARC和海马中观察到。结果：Ghrelin或者ARC电刺激可以兴奋海马区的胃牵张敏感神经元。Ghrelin受体拮抗剂[d-Lys-3]-GHRP-6预处理可以完全或者部分阻断这种兴奋作用。海马注射ghrelin可以显著促进胃运动，并且呈现剂量依赖关系，而且这种作用可以被[d-Lys-3]- GHRP-6所阻断。电刺激ARC能够促进胃运动。然而，预处理时[d-Lys-3]- GHRP-6可以减弱这些作用。电损毁海马可以减弱胃运动的兴奋作用，这个作用通过电刺激ARC产生的。结论：通过海马促进胃运动中ghrelin起着重要的作用。ARC可能参与调节海马对胃动力的影响。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the growth hormone secretagogue receptor 1A (GHSR-1a) expression in the hippocampus; and to explore the influence of ghrelin on hippocampus discharge GD sensitive neurons; explored the neural pathway between ARC and ghrelin neurons in the hippocampus, and the role of ARC in regulating hippocampal and gastric motility in discharge. Methods: The dis- charge of hippocampus was recorded in vitro and the gastric motility was measured in conscious rats. The expression of GHSR-1a in hip- pocampus was detected by PCR Western blot and immunofluorescence histochemical staining. The projection of ghrelin neurons was de- tected by retrograde tracing and immunofluorescence histochemical staining. Ghrelin - double labeled neurons and GHSR-1a expression were observed in ARC and hippocampus. Results: Ghrelin or ARC electrical stimulation could stimulate gastric stretch sensitive neurons in hippocampus. Pretreatment with the Ghrelin antagonist [d-Lys- 3]-GHRP-6 can completely or partially block the excitatory effect. Hip- pocampal injection of ghrelin can significantly promote gastric motility, and dose dependently, and this effect can be blocked by [d-Lys-3]- GHRP-6. Electrical stimulation of ARC can promote gastric motility. However, pretreatment with [d-Lys-3]- GHRP-6 can at- tenuate these effects. Electrical damage to the hippocampus can attenuate the gastric motility, which is produced by electrical stimulation of ARC. Conclusion: Ghrelin plays an important role in promoting gastric motility in hippocampus. ARC may be involved in the regula- tion of hippocampal motility. |
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