| 周 桑,吴 豫,宋婧文,沈 茜,赵仙先,秦永文.三卡因浓度对斑马鱼心脏手术模型麻醉的影响[J].现代生物医学进展英文版,2018,(5):822-825. |
| 三卡因浓度对斑马鱼心脏手术模型麻醉的影响 |
| Effects of Three-cardin Concentration on Anesthesia in Zebrafish Cardiac Surgery |
| Received:October 07, 2017 Revised:October 30, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.05.005 |
| 中文关键词: 斑马鱼 心脏 三卡因 麻醉 MS-222 |
| 英文关键词: Zebrafish Heart Traine Anesthesia MS-222 |
| 基金项目:国家自然科学基金项目（81470407） |
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| 中文摘要: |
| 摘要 目的：探讨斑马鱼麻醉过程中三卡因浓度对其心脏手术后生存率的影响。方法：以经典实验方法中的三卡因(MS-222)浓度150 mg/L为对照，分别用浓度为20 mg/L、40 mg/L、60 mg/L、80 mg/L、100 mg/L、150 mg/L、200 mg/L 的MS-222麻醉液麻醉，观察斑马鱼术后行为及术后生存率的差异。结果：随着MS-222浓度的提高，斑马鱼表现为入麻时间的逐渐减少、苏醒时间的逐渐增加。MS-222麻醉斑马鱼成鱼的有效浓度为40～160 mg/L，在此浓度范围内，鱼体能够在3 min内达到可施行手术操作的麻醉状态(Ⅱ或Ⅲ期)，10 min内可苏醒恢复；随着浓度的增大，呼吸频率的下降速率增大；MS-222浓度为40 mg/L时斑马鱼的术后存活率最高(98.3%)。结论：当MS-222浓度为40 mg/L时，斑马鱼入麻及苏醒较为平稳，术后斑马鱼复苏时间较短，且保持了较高的术后生存率。在实验操作或者生产应用时推荐MS-222麻醉药液最佳麻醉浓度为40 mg/L，所对应的麻醉时间约为15 min左右。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of tristate concentration on the survival rate of zebrafish after partial cardiac resec- tion. Methods: The concentration of tricaine (MS-222) anesthetic liquid in the classical experimental method is 150 mg/L. Zebrafish were treated with MS-222 at different concentrations including 20 mg/L, 40 mg/L, 60 mg/L, 80 mg/L, 100 mg/L, 150 mg/L and 200 mg/L to observe the different postoperative behavior and postoperative survival rate. Results: It was found that at a higher concentration zebrafish exhibit a remarkably long recovery time and a reduction of anesthesia time. The effective MS-222 concentration of zebrafish adult fish is 40-160 mg/L. Zebrafish can reach the anesthetic status (Ⅱ or Ⅲ) suitable for the operation within 3min, and recover within 10min. With the increase of the concentration, the decrease rate of the respiratory rate increases and the survival rate of zebrafish is the highest (98.3%) at the concentration of 40 mg/L. Conclusion: The results showed that when the concentration of MS-222 was 40 mg/L, zebrafish recovered faster with higher recovery ratio. In the experimental operation or production applications, MS-222 anesthetic concentration of 40 mg/L is recommended and the corresponding anesthesia time is about 15 minutes. |
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