| 王兆宁,郑立峰,陈 岩,王 宝,王 可,王居正.NOK在骨肉瘤细胞系MG63 EMT过程中的作用[J].现代生物医学进展英文版,2018,(3):438-441. |
| NOK在骨肉瘤细胞系MG63 EMT过程中的作用 |
| The Roles of NOK in the EMT Process of Osteosarcoma |
| Received:August 28, 2017 Revised:September 22, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.03.008 |
| 中文关键词: NOK 骨肉瘤 EMT |
| 英文关键词: NOK Osteosarcoma EMT |
| 基金项目:国家自然科学基金项目（81572252） |
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| 中文摘要: |
| 摘要 目的：通过对比和检测人正常成骨细胞系hFOB1.19以及骨肉瘤细胞系MG63中NOK以及EMT标志性分子E-cadherin、Vimitin的mRNA和蛋白表达量，并观察NOK对骨肉瘤细胞系MG63中EMT标志性分子E-cadherin及Vimitin的mRNA和蛋白表达量的影响，探讨NOK在骨肉瘤细胞系MG63 EMT过程中的作用。方法：qRT-PCR、Western blot法检测人正常成骨细胞系 hFOB1.19以及骨肉瘤细胞系MG63中NOK、E-cadherin、Vimitin的mRNA和蛋白表达量；构建慢病毒并干扰骨肉瘤细胞系MG63中NOK表达，qRT-PCR、Western blot法检测干扰NOK前后EMT标志性分子E-cadherin及Vimitin的mRNA和蛋白表达。结果：相比于人正常成骨细胞系，NOK、Vimitin的mRNA和蛋白在骨肉瘤细胞系MG63中高表达，E-cadherin的mRNA和蛋白在骨肉瘤细胞系MG63中低表达。慢病毒干扰骨肉瘤细胞中NOK表达后，E-cadherin的mRNA和蛋白表达升高，Vimitin的mRNA和蛋白表达降低。结论：NOK具有促进骨肉瘤细胞系MG63发生EMT过程。 |
| 英文摘要: |
| ABSTRACT Objective: Through comparing and testing the mRNA and protein expressions of NOK and EMT related molecules E-cadherin and Vimitin in normal ossification cell lines hFOB1.19 and osteosarcoma cell lines MG63, and observing the influence of NOK on the expression of E-cadherin and Vimitin in osteosarcoma, and then the role and mechanism of NOK in the EMT process of os- teosarcoma will be explored. Methods: qRT-PCR and Western blot analysis were used to detect the NOK, E-cadherin and Vimitin mRNA and protein expression; and then build slow virus and RNA interference NOK expression in osteosarcoma cell lines MG63, and observe the E-cadherin and Vimitin mRNA and protein expression before or after lentivirus vectors for NOK RNA interfering through qRT-PCR and Western blot. Results: Comparing with normal ossification cell lines, the mRNA and protein of NOK and Vimitin are high expressed and E-cadherin mRNA and protein expression are lower in osteosarcoma cell line MG63. Lentivirus vectors interfering NOK could in- crease E-cadherin mRNA and protein expression, and reduce Vimitin mRNA and protein expression. Conclusion: NOK could promote the EMT process in osteosarcoma cell line MG63. |
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