| 凯赛尔江·多来提,古丽美热·艾买如拉,吐尔逊江·达地汗,迪力夏提·艾尼,廖礼彬,迪力木拉提·艾尼,李 杰,陈胜国,古丽尼沙·克力木.人类胚胎三叉神经节细胞的局在性分布[J].现代生物医学进展英文版,2018,(3):433-437. |
| 人类胚胎三叉神经节细胞的局在性分布 |
| A Study on the Distinct Distribution of Trigeminal Ganglion in Human Fetus |
| Received:July 22, 2017 Revised:August 15, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.03.007 |
| 中文关键词: 人类胚胎 DiI 三维重建 三叉神经节细胞 |
| 英文关键词: Human fetus DiI Three bit reconstruction Trigeminal ganglion cell |
| 基金项目:国家自然科学基金项目(31360284) |
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| 中文摘要: |
| 摘要 目的：应用三维重建方法对人类胚胎三叉神经节细胞的局在性分布进行研究。方法：本研究选用非疾病死亡的引产胚胎标本8例，胎龄20-26周，在获取标本1-4小时内，对标本进行灌流固定。其中2例标本在手术显微镜下开颅取出三叉圣经节，石蜡包埋、冰冻切片、HE染色、光学显微镜下观察、照相，用三位重建技术制作三维立体图片。其余6例标本在手术显微镜下开颅、找出三叉神经三大分支眼神经、上颌神经及下颌神经，各选2例分别注入DiI结晶体、在37 ℃恒温箱内保存3个月，取出标本、明胶包埋、冰冻切片，在荧光显微镜下观察、照相，用三位重建技术制作三维立体图片。结果：①眼神经的节细胞分布于神经节的前内侧、下颌神经的节细胞在神经节的后外侧、上颌神经的节细胞位于眼神经和下颌神经节细胞之间。②上颌神经和下颌神经节细胞之间存在少量的重叠。结论：三叉神经节细胞在神经节内由前内侧向后外侧分别为眼神经、上颌神经、下颌神经的顺序排列；上颌神经和下颌神经的起始细胞之间存在少量的重叠现象；三维重建图片结果显示人胚胎三叉神经节细胞即眼神经、上颌神经及下颌神经的起始细胞存在明显的局在性分布特征。 |
| 英文摘要: |
| ABSTRACT Objective: To study the distinct distribution of trigeminal ganglion cells in human fetus by three bit reconstruction technique. Methods: 8 cases of human embryonic specimens which non-disease deaths of the fetus induced labor, gestational age 20 -26weeks, obtaining the specimen within 1 to 4 hours, and these specimens were fixed. 2 specimens, taking out the trigeminal ganglion under the operation microscope, and the paraffin embedding, frozen section, HE staining, and take photos under optical microscope and making 3D pictures with three bit reconstruction technique. In the rest 6 specimens, under operation microscope, finding out ophthalmic nerve,maxillary nerve, mandibular nerve and inject with DiI respectively, to keep 3 months in incubator at 37 ℃, and to take out these specimens, gelatin embedding, frozen section and take photos under the fluorescence microscope and making 3D pictures with three bit reconstruction technique. Results: ①The ganglion cells of the ophthalmic nerve are located in the anteromedial region of the trigeminal ganglion, the ganglion cells of the mandibular nerve are located in the posterolateral region of the trigeminal ganglion, the ganglion cells of the maxillary nerve lie between the ophthalmic and mandibular ganglion cells.②There is a small amount of overlap between the max- illary and the mandibular ganglion cells. Conclusion: The trigeminal ganglion cells are arranged sequentially from the anteromedial to posterolateral, the ophthalmic, maxillary and mandibular nerves respectively in the trigeminal ganglion; There is a small amount of over- lap between the initial cells of maxillary nerve and the mandibular nerve; These three dimensional reconstruction pictures indicate the trigeminal ganglion cells in human fetus, the ophthalmic nerve, maxillary nerve and mandibular nerve initiating cells have the characteris- tics of obvious distinct distribution. |
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