| 马骏锴,倪国莉,李西林,何 洋,周绍兵,杨 光.一种具有空腔结构的高分子超细纤维的制备及应用研究[J].现代生物医学进展英文版,2018,(3):413-418. |
| 一种具有空腔结构的高分子超细纤维的制备及应用研究 |
| The Preparation and Application of Polymer Superfine Fiber with Cavities |
| Received:May 30, 2017 Revised:June 25, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.03.003 |
| 中文关键词: 静电纺丝技术 微流控技术 高分子 超细纤维 |
| 英文关键词: Electrospinning Microfluidic technology Polymer Superfine fiber |
| 基金项目:国家自然科学基金项目（51173150）；国家重大科学研究计划（973计划）子课题（2012CB933602）；高等学校博士学科点专项科研基金项目（20120184110029） |
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| 中文摘要: |
| 摘要 目的：制备一种具有空腔结构的高分子超细纤维并研究其相关性质，探索其应用。方法：结合静电纺丝技术和微流控技术，制备出具有空腔结构的聚乳酸（PLA）超细纤维，并使用荧光显微镜、扫描电子显微镜、透射电子显微镜等手段进行结构表征；采用甘油铜分光光度法、Alamar-Blue法间接检测了纤维膜的甘油含量和细胞毒性，并考察了其吸水率相较于普通实心纤维膜的变化；将PEI-质粒复合物载入纤维的空腔结构中，通过细胞转染实验验证了此纤维膜在运载质粒方面的应用。结果：纤维平均直径在1 μm左右，内部均匀分布着椭圆形空腔。该纤维膜中甘油占比38.99 %，吸水率为普通实心PLA纤维膜的近2倍。纤维膜与内皮细胞5天的共培养中，没有明显的细胞毒性。细胞转染检测结果证明了纤维空腔部分能有效运载质粒复合物并保证其生物活性。结论：静电纺丝技术和微流控技术有效结合，成功制备出具有空腔结构的新型高分子超细纤维，展现出了区别于普通纤维的独特性质和应用。 |
| 英文摘要: |
| ABSTRACT Objective: To prepare a novel polymer superfine fiber with internal cavities and its properties and applications are re- searched. Methods: The novel polymer superfine fiber with cavities was prepared by combining electrospinning and microfluidic technol- ogy and then was characterized by fluorescence microscope, scanning electron microscope (SEM) and transmission electron microscopy (TEM); The glycerol content and cytotoxicity of this fiber membrane were investigated by cupric glycerinate spectrophotometry and Ala- mar-Blue assay. Besides, the difference about water absorption between the fiber and the conventional solid one was compared. The PEI-plasmid complexes were loaded into the cavities of the fiber and its application in transporting plasmids was studied by cell transfec- tion experiments. Results: The fiber diameter was about 1 μm and the elliptical cavities were orderly distributed inside. The mass frac- tion of glycerol in the membrane was 38.99 % and water absorption of the membrane was nearly as twice as the conventional one without cavities. During the co-culture with endothelial cells for 5 days, the membrane had not shown significant cytotoxicity. Besides, the cavi- ties inside fiber could effectively carry the PEI-plasmid complexes and maintain their biological activity, as verified by cell transfection experiments. Conclusion: Electrospinning and microfluidic technology were successfully combined to prepare a polymer superfine fiber with cavities and this fiber membrane exhibited unique properties and might be promising in many applications. |
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