王 顺,王 唯,杨 针,夏聪聪,刘 磊,李海民.OIP5对肝癌细胞SMMC-7721增殖和侵袭迁移能力的影响[J].现代生物医学进展英文版,2018,(2):215-218. |
OIP5对肝癌细胞SMMC-7721增殖和侵袭迁移能力的影响 |
Effects of OIP5 on the Proliferation, Migration and Invasion of Hepatocellular Carcinoma Cell line SMMC-7721 |
Received:June 23, 2017 Revised:July 18, 2017 |
DOI:10.13241/j.cnki.pmb.2018.02.004 |
中文关键词: OIP5 原发性肝癌 增殖 侵袭 迁移 |
英文关键词: OIP5 Hepatocellular carcinoma Proliferation Migration Invasion |
基金项目:国家自然科学基金项目(81672339) |
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中文摘要: |
摘要 目的:探讨OIP5对肝癌细胞SMMC-7721增殖和侵袭迁移能力的影响。方法:采用RNA干扰技术沉默肝癌细胞中OIP5的表达后,通过qRT-PCR和Western-blot技术检测OIP5的下调效率,CCK-8和平板克隆法检测肝癌细胞的增殖能力,Transwell法检测肝癌细胞的侵袭和迁移能力。结果:转染OIP5-siRNA后,肝癌细胞SMMC-7721中OIP5 mRNA和蛋白的表达水平均明显降低(P<0.05);同时,与对照组相比,OIP5-siRNA组肝癌细胞SMMC-7721的CCK-8实验的OD值、平板克隆法测得的克隆球个数、Transwell法测得的迁移细胞数与侵袭细胞数均明显低于对照组(P<0.05)。结论:OIP5能够促进肝癌细胞的增殖和侵袭迁移,可能作为肝癌治疗的潜在靶点。 |
英文摘要: |
ABSTRACT Objective: To investigate the protective effects of OIP5 on the proliferation, migration and invasion of hepatocellular carcinoma(HCC) cell line SMMC-7721. Methods: OIP5 was down-regulated by using small interfering RNA in HCC cell line SMMC-7721. The transfection efficiency of OIP5-siRNA was detected with qRT-PCR and Western blot. The proliferation of HCC cells was detected by CCK-8 and colony formation assay, and the migration and invasion of HCC cells were measured by Transwell assay. Results: The level of OIP5 mRNA and protein were significantly decreased after being transferred by OIP5-siRNA in SMMC-7721 cells. After knockdown of OIP5, the OD value measured by CCK-8, the number of conoly, metastasis and invaded cell number were significantly decreased compared with the control group (P<0.05). Conclusion: OIP5 can promote the proliferation, invasion and metastasis of HCC SMMC-7721 cells, and targeting OIP5 is likely to be a promising strategy for the treatment of HCC. |
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