| 凯赛尔江·多来提,古丽美热·艾买如拉,吐尔逊江·达地汗,陈胜国,廖礼彬,迪力夏提·艾尼,迪力木拉提·艾尼,李 杰,古丽尼沙·克力木.鱼类三叉神经中脑核位置及细胞形态的研究[J].现代生物医学进展英文版,2018,(1):32-36. |
| 鱼类三叉神经中脑核位置及细胞形态的研究 |
| A Study on the Location and Cell Morphology of Mesencephalic Trigeminal Nucleas in Tilapia |
| Received:May 23, 2017 Revised:June 16, 2017 |
| DOI：10.13241/j.cnki.pmb.2018.01.007 |
| 中文关键词: 三叉神经中脑核 生物胞素 鱼类 |
| 英文关键词: Mesencephalic trigeminal nucleas Biocytin Tilapia |
| 基金项目:国家自然科学基金项目(31360284) |
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| 中文摘要: |
| 摘要 目的：应用生物胞素法观察罗非鱼三叉神经中脑核位置及细胞形态特征。方法：本研究选用罗非鱼15 只(雌雄不限)，体长 15～20 cm，浸入140 mg/L 三卡因间氨苯酸乙脂甲磺酸盐(MS222)溶液中麻醉，在手术显微镜下暴露脑和神经，通过生物胞素（Biocytin）结晶追踪技术研究罗非鱼三叉神经中脑核的位置、细胞形态分布。结果：①罗非鱼的三叉神经中脑核位于中脑后交联水平。②三叉神经中脑核的下行纤维与运动核之间存在突触联系。③三叉神经中脑核的细胞形态为圆形或卵圆形。结论：罗非鱼的三叉神经中脑核位于中脑后交联的高度，其接受走行于三叉神经三大分支内的感觉纤维并发出的下行纤维与运动核形成突出联系。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the location and morphology of mesencephalic trigeminal nucleas in tilapia by Biocytin tracing method. Methods: This experiment with tilapia, 15( male or female), body length 15-20 cm, tilapias were deeply anesthetized by immer- sion in a solution of approximately140 mg/L{tricaine methanesulfonate (MS222)}, then the brain and nerves were exposed under surgical microscope and the location and morphology of mesencephalic trigeminal nucleas in tilapia was investigated by Biocytin tracing method. Results: ①Mesencephalic trigeminal nucleas in tilapia located in posterior position of midbrain of crosslinking level. ②Between descend- ing fibers of mesencephalic trigeminal nucleas and motor nucleas has synaptic connections in tilapia. ③The morphology of mesencephalic trigeminal nucleas is round or oval shape. Conclusion: Mesencephalic trigeminal nucleas in tilapia located in posterior position of mid- brain of crosslinking level. Mesencephalic trigeminal nucleas can accepts the sensory fibers of the three branches of trigeminal nerve and between descending fibers of mesencephalic trigeminal nucleas and motor nucleas has synaptic connections in tilapia. |
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