| 王博文,孙永涛,康 文.基因编辑在艾滋病治疗中的研究进展[J].现代生物医学进展英文版,2017,17(35):6984-6987. |
| 基因编辑在艾滋病治疗中的研究进展 |
| Gene Therapy Strategies for the Treatment of HIV/AIDS |
| Received:December 07, 2016 Revised:December 31, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.35.043 |
| 中文关键词: 艾滋病 基因编辑 ZFN CRISPR NgAgo |
| 英文关键词: AIDS Gene therapy ZFN CRISPR NgAgo |
| 基金项目:国家"十二五"科技重大专项（2014ZX10001002）；国家自然科学基金青年基金项目（81501041） |
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| 中文摘要: |
| 摘要：随着HIV病毒学研究的不断深入，传统抗反转录病毒治疗在HIV病毒清除和疾病治愈中的局限性日益凸显，因此通过基因编辑达到HIV病毒控制甚至完全治愈的策略倍受重视。目前基因编辑的研究主要包括以下两个方面：RNA水平的编辑技术和DNA水平的编辑技术。近年来包括锌指核酶技术（zinc finger nucleases， ZFN），转录激活样效应因子技术（transcription activator- like effector nucleases， TALEN），成簇规律间隔短回文重复序列技术（clustered regulatory interspaced short palindromic repeat， CRISPR）和NgAgo-gDNA技术在内的DNA编辑技术进展迅猛，并在HIV治疗中取得了长足的发展。本文比较了不同DNA水平编辑方法的特点和差异，综述了新一代基因编辑技术在HIV治疗中的进展以及潜在的医学应用前景。 |
| 英文摘要: |
| ABSTRACT: With the further understanding of the molecular virological mechanisms of HIV pathogenesis, the limitation of tradi- tional antiretroviral therapy for a cure of HIV is appearing. Therefore, gene therapy has attracted more and more attention. Currently, two kinds of gene editing strategies for gene therapy were based on RNA and DNA respectively. The latter includes ZFN(zinc finger nucleases), TALEN(transcription activator-like effector nucleases), CRISPR(clustered regulatory interspaced short palindromic repeat) and NgAgo- gDNA, which lead the development of gene editing and achieve great success in HIV therapy. In this review, we first compare the spe- cialty and distinction between different DNA editing methods, and then summarize the advances and prospects of these new methods in HIV gene therapy. |
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