| 张姝江,陈 艺,姚咏嫦,谭帼馨,施雪涛.用于组织工程软骨支架的改性水凝胶初步筛选[J].现代生物医学进展英文版,2017,17(35):6813-6817. |
| 用于组织工程软骨支架的改性水凝胶初步筛选 |
| Preliminary Screening of Modified Hydrogels for Tissue Engineering Cartilage Scaffold |
| Received:June 21, 2017 Revised:July 13, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.35.004 |
| 中文关键词: 软骨 组织工程 生物相容性材料 干细胞 |
| 英文关键词: Cartilage Tissue engineering Biocompatible materials Stem cells |
| 基金项目:广东省自然科学基金杰出青年基金项目（2016A030306018）；广州市属高校科研项目重点项目（1201610097） |
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| 中文摘要: |
| 摘要 目的：本研究对水凝胶材料加入不同复合成分进行改性后，通过细胞在材料表面的生长增殖情况，筛选适宜作为组织工程软骨支架的复合材料。方法：PEGD水凝胶中分别以8:2和6:4两种比例复合NVP或HEMA；大鼠骨髓基质干细胞（MSCs）接种到改性后的复合材料表面共培养。以扫描电子显微镜（SEM）观察细胞在复合材料表面的生长状况。根据SEM观察结果，选择适宜的复合材料成分及比例，分别以等离子处理、丙烯酰胺接枝、真空干燥法和冻干法处理材料表面，再次接种MSCs，并以扫描电镜观察细胞生长；适宜材料在接种细胞前后的抗压强度也进行了评价。结果：根据SEM结果，(8:2)的PEGDA/HEMA 和PEGDA/ NVP表面完全无细胞生长；而在6:4比例的PEGDA/HEMA 和PEGDA/NVP材料表面MSCs都有生长，其中以PEGDA/HEMA细胞增殖更明显。选择(6:4)PEGDA/HEMA复合材料分别进行三种方式表面处理，MSCs接种后在各种表面处理方式的材料都有生长，但以等离子处理后的材料表面细胞生长最旺盛，形态最好。细胞在(6:4)PEGDA/HEMA表面复合培养前后，材料的抗压强度没有统计学意义上的显著差别（P＞0.05）。结论：PEGDA/HEMA(6:4)具有良好的细胞相容性，尤其经等离子处理表面后细胞生长更优，可以作为组织工程软骨的支架材料。 |
| 英文摘要: |
| ABSTRACT Objective: To make a quick screening of the hydrogels with different compositions for cartilage tissue engineering. Methods: Compound materials of PEGD/ NVP and PEGD/HEMA in two different portions (8:2 and 6:4) were co-cultured with the MSCs which were seeded onto the materials. SEM observation was performed, and the composite of the appropriate portion and component was chosen which was then treated by plasma surface modification, vacuum dehydration, or lyophilization respectively. The cytocompability of the material was evaluated together with the mechanical characteristics. Results: No cell adhesion and proliferation was observation the surface of PEGDA/HEMA(8:2) nor the surface of PEGDA/NVP(8:2) according to SEM. The cells on both PEGDA/HEMA(6:4) and PEGDA/NVP(6:4) grew and spread well, yet the extracellular matrix deposited better on the former than the latter. Thus PEGDA/HEMA(6:4) was chosen to be treated by the three different modification progresses respectively. The SEM demonstrated that the cells grew well with obvious proliferation and fusing together on the three types of modified PEGDA/HEMA(6:4); large amount of extracellular matrix deposition could also be seen. The PEGDA/HEMA(6:4) modified by plasma showed a much better cell morphology. No significant change has been found in the compression stress of PEGDA/HEMA(6:4). Conclusion: PEGDA/HEMA(6:4) has a good cytocompatibility, and is especially good for the cell growth after plasma surface modification which makes it a potential biomaterial for the scaffold of tis- sue engineering cartilage. |
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