| 夏 力,董 翔,康 燕,沈才福,马 娜,宋来阳,刘江伟.姜黄素预处理对沙漠干热环境热射病大鼠肠黏膜的损伤保护作用及对TLR4/NF-κB信号通路的影响[J].现代生物医学进展英文版,2017,17(34):6648-6652. |
| 姜黄素预处理对沙漠干热环境热射病大鼠肠黏膜的损伤保护作用及对TLR4/NF-κB信号通路的影响 |
| Protective Effects of Curcumin on Intestinal Mucosa Injury of Heatstroke Rats in the Dry Heat Envrionment of Desert and Its Effects on TLR4/NF-κB Signaling Pathway |
| Received:August 10, 2017 Revised:September 06, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.34.009 |
| 中文关键词: 沙漠 干热环境 热射病 肠黏膜 Toll样受体4 核因子-κB 信号通路 |
| 英文关键词: Dry-heat environment Desert Heatstroke Intestinal mucosa TLR4 NF-κB Signaling pathway |
| 基金项目:新疆自治区自然科学基金项目(2015211C231) |
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| 中文摘要: |
| 摘要 目的：探讨姜黄素预处理对沙漠干热环境热射病大鼠肠黏膜损伤保护作用及对TLR4/NF-κB信号通路的影响。方法：随机将40只雄性SD大鼠分为5组，常温对照组（NC组）、干热对照组（DH组）、低剂量姜黄素预处理组（LDC组）、中剂量姜黄素预处理组（MDC组）、高剂量姜黄素预处理组（HDC组）。NC组和DH组生理盐水灌胃，其他三组按（50、100、200）mg/kg姜黄素灌胃，连续7天。将DH组和姜黄素各组置于西北特殊环境人工实验舱中，设置温度：（41±0.5）℃，湿度：（10±1）%RH，干热暴露150 min建立热射病（重度中暑）模型。麻醉处死后采集回肠组织样本，HE染色观察肠黏膜形态学变化并进行病理损伤评分，Western blotting检测肠黏膜Toll样受体4（TLR4）、核因子-κB （NF-κB）及其抑制蛋白（IκB）的表达。结果：DH组肠粘膜病理损伤评分较NC组显著升高（P< 0.05）；各姜黄素预处理组病理损伤评分较DH组显著降低（P< 0.05）。DH组较NC组TLR4、NF-κB表达显著升高，IκB表达显著降低（P< 0.05）；各姜黄素预处理组较DH组TLR4、NF-κB表达降低，而IκB表达增加，具有显著性差异（P< 0.05）。结论：姜黄素能够减轻沙漠干热环境热射病大鼠肠黏膜病理学损伤，其机制可能与姜黄素抑制TLR4/NF-κB信号通路有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the mechanism of protective effects of curcumin on intestinal mucosa injury of heatstroke rats in the dry heat envrionment of desert and its effects on TLR4/NF-κB signaling pathway. Methods: 40 male SD rats were randomly divided into 5 groups, normal temperature control group (NC group), dry heat control group (DH group), low dose curcumin pretreated group (LDC group), middle dose curcumin pretreated group (MDC group) and high dose curcumin pretreated group (HDC group). NC and DH group were gavaged with saline. Each of curcumin pretreated groups were gavaged with (50, 100, 200)mg/kg curcumin respectively, once a day for 7days. The DH and each curcumin groups were palced in The Simulated Climate Cabin for Special Environment of Northwest of China, the environment temperature was set to (41±0.5)℃, and humidity was to (10±1)%RH, Rats were exposed to dry-heat environment for 150 min, resulted in severe heatstroke models. The samples of ileum were collected after put to death under anesthesia. HE staining was used to observe the morphological changes of the intestinal mucosa and the pathological damage were scored. The expression of TLR4, IκB, NF-κB protein in intestinal mucosa was detected by Western blotting. Results: The pathological injury score of DH group were significantly higher than that of NC group(P < 0.05), the pathological injury score of curcumin groups were significantly lower than that of DH group(P < 0.05). The expression of TLR4 and NF-κB in DH group was significantly increased compared with that of NC group, however, the expression of IκB significantly decreased (P <0.05), curcumin groups compared with DH group the expression of TLR4 and NF-κB in each curcumin pretreated group significantly decreased compared with that of the DH group, however, the expression of IκB significantly increased(P < 0.05). Conclusion: Curcumin can alleviate the pathological damage of intestinal mucosa of rats with severe heat stroke in dry-heat environment of desert, the mechanism may be related to the inhibition of TLR4/NF-κB signaling pathway by curcumin. |
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