| 楚 轶,冯 品,党晶艺,张 薇,曾 迪.全身稳恒磁场暴露对糖尿病动脉粥样硬化大鼠血浆和主动脉中重要细胞因子表达的调控作用[J].现代生物医学进展英文版,2017,17(34):6620-6625. |
| 全身稳恒磁场暴露对糖尿病动脉粥样硬化大鼠血浆和主动脉中重要细胞因子表达的调控作用 |
| Regulatory Effect of Whole-body Exposure to Static Magnetic Fields on Important Blood and Aortic Cytokine Expression in Diabetic Atherosclerosis Rats |
| Received:July 27, 2017 Revised:August 20, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.34.004 |
| 中文关键词: 稳恒磁场 糖尿病 动脉粥样硬化 细胞因子 炎症反应 |
| 英文关键词: Static magnetic fields Diabetes mellitus Atherosclerosis Cytokines Inflammatory response |
| 基金项目:国家自然科学基金面上项目(81470537) |
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| 中文摘要: |
| 摘要 目的：探讨中等强度(4.0mT)的全身性稳恒磁场暴露对于糖尿病动脉粥样硬化大鼠血浆和主动脉中的重要细胞因子表达的调控作用。方法：将30只12周龄的雄性SD大鼠随机等分至空白对照组(Control)、糖尿病组(DM)及糖尿病磁场刺激组(DM+SMF)，每组10只大鼠。DM组和DM+SMF组的大鼠采用链脲佐菌素STZ+维生素D3+高脂饮食协同作用法建立糖尿病性动脉粥样硬化模型，DM+SMF组的大鼠接受强度4.0mT全身稳恒磁场暴露，每天刺激2小时，连续刺激8周。Control组大鼠不施加任何药物或磁场干预，作为空白对照。8周后，处死全部大鼠，提取血液样本，检测血脂四项(血清总胆固醇、甘油三酯、高密度脂蛋白胆固醇以及低密度脂蛋白胆固醇)，并使用ELISA法检测血清血管内皮生长因子(VEGF)、肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)及白细胞介素6(IL-8)水平。使用PCR法检测主动脉中VEGF、TNF-α、IL-1、IL-6、半胱氨酸的天冬氨酸蛋白水解酶1(Caspase 1)以及NOD样受体蛋白3炎症小体(NLRP3)的mRNA表达。结果：DM+SMF大鼠血清总胆固醇、血清甘油三酯、低密度脂蛋白和高密度脂蛋白含量显著低于DM组(P<0.05)，血清VEGF、TNF-α、IL-1β、IL-6及IL-8蛋白表达及其在主动脉组织中mRNA表达、主动脉组织中Caspase 1和NLRP3mRNA表达均显著减少(P<0.05)。结论：中等强度(4 mT)的稳恒磁场刺激对糖尿病性动脉粥样硬化发生和发展的对抗作用可能与其对重要细胞因子(如VEGF、TNF-α、IL-1β、IL-6、IL-8、Caspase 1及NLRP3)的表达调控作用相关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the potentially regulatory effects of whole-body exposure to moderate-intensity (4.0 mT) static magnetic fields on the important blood and aortic cytokine expression in diabetic atherosclerosis rats. Methods: Twelve-week-old male SD rats (n=30) were randomly and equally assigned into 3 groups with 10 rats in each group, including the blank control (Control), diabetes mellitus (DM) and diabetes mellitus with static magnetic fields exposure (DM+SMF) groups. The method of combined administration with streptozotocin, vitamin D3 and high-fat diet was employed to induce diabetic atherosclerosis model for the rats in the DM and DM+SMF groups. Rats in the DM+SMF group were subjected to whole-body stimulation with static magnetic fields (4.0 mT) for 2 hours per day with a consecutive 8-week exposure period. Rats in the Control group were not subjected to any drug or magnetic fields stimulation for acting as the blank control. After 8 weeks, all rats in the three groups were sacrificed and the blood samples were collected for the blood lipid parameter analysis, including serum total cholesterol, triglyceride, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol. Furthermore, the ELISA approach was employed to quantify the serum vascular endothelial growth factor (VEGF), tumor necrosis factor α (TNF-α), interleukin 1 (IL-1β), interleukin 6 (IL-6) and interleukin 8 (IL-8) levels. Moreover, the aortic tissues were also extracted for determining the VEGF, TNF-α, IL-1β, IL-6, cysteinyl aspartate specific proteinase 1 (Caspase 1), and nod-like receptor pyrindomain-containing protein 3 (NLRP3) mRNA expression via the PCR approach. Results: Rats in the DM+SMF group exhibited significantly lower serum total cholesterol, triglyceride, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol than the DM group (P<0.05). Moreover, the DM+SMF group also showed significant decrease in serum VEGF, TNF-α, IL-1β, IL-6, and IL-8 protein expression and aortic VEGF, TNF-α, IL-1β, IL-6, Caspase 1 and NLRP3 mRNA expression as compared with the DM group (P<0.05). Conclusion: The resistive effects of moderate-intensity static magnetic fields on the occurrence of development of diabetic atherosclerosis are associated with their regulation of essential cytokines, such as VEGF, TNF-α, IL-1β, IL-6, IL-8, Caspase 1 and NLRP3, etc. |
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