| 王 颖,戚 瑞,张 磊,王晓慧,徐孝娜,谢祥军,杨瑞华.鱼油对高脂喂养加小剂量STZ诱导的2型糖尿病大鼠血脂及肝脏的影响[J].现代生物医学进展英文版,2017,17(33):6406-6411. |
| 鱼油对高脂喂养加小剂量STZ诱导的2型糖尿病大鼠血脂及肝脏的影响 |
| Effect of Fish Oil on the Lipid Metabolism of Liverin High Fat Diet Plus Low-dose STZ induced Type 2 Diabetic Rats |
| Received:July 23, 2017 Revised:August 18, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.33.002 |
| 中文关键词: 高脂饮食 脂代谢 2型糖尿病 鱼油 氧化应激 |
| 英文关键词: High-fat diet Fat metabolism Type 2 diabetes Fish oil Oxidative stress |
| 基金项目:国家自然科学基金面上项目(81271194)；陕西省自然科学基金项目(2011JM4002) |
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| 中文摘要: |
| 摘要 目的：探讨鱼油对高脂喂养加小剂量STZ诱导的2型糖尿病大鼠脂代谢及肝脏组织的影响及其机制。方法：Sprague-Dawley大鼠随机分为对照组（Control）、高脂组（HF）、糖尿病组（DM）、高脂鱼油组（HF+FO）和糖尿病鱼油组（DM+FO），观察大鼠周体重变化，检测各组大鼠血糖、血脂、肝脏细胞形态学及过氧化损伤情况，探讨其可能的作用机制。结果：与对照组相比，高脂喂养大鼠体重明显增加（387.74±36.20 g vs 339.64±10.41 g, P<0.05），血甘油三酯(triglyceride, TG)、总胆固醇（total cholesterol, TC）和低密度脂蛋白胆固醇(low density lipoprotein cholesterol, LDL-C)分别由1.96±0.06 mmol/L、1.91±0.09 mmol/L和0.72±0.07 mmol/L升高至2.29±0.16 mmol/L、2.08±0.06 mmol/L和1.22±0.09 mmol/L，高密度脂蛋白胆固醇(high density lipoprotein cholesterol, HDL-C)降低（0.92±0.15 mmol/L vs 1.80±0.07 mmol/L，P<0.05），膳食添加鱼油后均有明显改善。糖尿病大鼠体重减轻（287.78±16.11 g vs 339.64±10.41 g, P<0.05）；但血清中LDL-C水平升高至1.08±0.08 mmol/L，HDL-C水平降低至1.09±0.07 mmol/L（P<0.05），鱼油可显著提高HDL-C的水平（P<0.05）。高脂组和糖尿病组均有明显的肝脏脂肪浸润和过氧化损伤，添加鱼油后明显减轻。结论：膳食添加鱼油对高脂膳食及2型糖尿病引起的血脂异常均有保护作用，可降低血中甘油三酯和低密度脂蛋白胆固醇水平；减轻大鼠肝脏脂肪浸润和过氧化损伤。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of fish oil on the lipid metabolism and liver injury of high fat diet and low-dose strep- tozotocin (STZ) induced type 2 diabetic rats. Methods: Sprague-Dawley rats were randomly divided into control group (Control), high fat group (HF), diabetes mellitus group (DM), high fat + fish oil group (HF + FO) and diabetes mellitus + fish oil group (DM + FO). Body weight changes were detected weekly, and blood sugar, blood fat, liver cell morphological changes and hyperoxidative injury were de- tected at the beginning and end of the experimental period. Further explore the mechanisms of injury and effect of fish oil. Results: Com- paring with control group, body weight of high fat diet rats increased（387.74±36.20 g vs 339.64±10.41 g, P<0.05）. High fat diet in- duced triacylglyceride (TG), total cholesterol（TC） and low density lipoprotein-cholesterol (LDL-C) increase from 1.96±0.06 mmol/L, 1.91±0.09 mmol/L and 0.72±0.07 mmol/L to 2.29±0.16 mmol/L, 2.08±0.06 mmol/L and 1.22±0.09 mmol/L, respec- tively. High density lipoprotein-cholesterol (HDL-C) decrease in rats（0.92±0.15 mmol/L vs 1.80±0.07 mmol/L, P<0.05）. Dietary fish oil reversed the changes significantly. The body weight of diabetic rats decreased（287.78±16.11 g vs 339.64±10.41 g, P<0.05）. The serum level of LDL-C increased to 1.08±0.08 mmol/L, and HDL-C decreasedto 1.09±0.07 mmol/L（P<0.05）. Fish oil supplementation increased HDL-C in diabetic rats（P<0.05）. Fatty infiltration and peroxidative injury of liver were observed in both HF and DM rats, and dietary fish oil improved the injury. Conclusion: Dietary fish oil has protective effect on hyperlipidemia in high fat diet and type 2 diabetes melli- tus rats. It can decrease blood TG and LDL-C level and reduce the fatty infiltration and oxidative injury of liver. |
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