| 祝勇刚,邹继伟,赵广跃,杨 照,李 军.低浓度万古霉素复合PMMA骨水泥的细胞毒性研究[J].现代生物医学进展英文版,2017,17(32):6247-6251. |
| 低浓度万古霉素复合PMMA骨水泥的细胞毒性研究 |
| Study on Cytotoxicity of Low Concentration Vancomycin/PMMA Composite Cements |
| Received:August 17, 2017 Revised:September 10, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.32.009 |
| 中文关键词: 万古霉素 聚甲基丙烯酸甲酯 细胞毒性 骨水泥 |
| 英文关键词: Vancomycin PMMA Cytotoxicity Bone cement |
| 基金项目:国家自然科学基金项目(81301535) |
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| 中文摘要: |
| 摘要 目的：研究低浓度万古霉素(1%)复合聚甲基丙烯酸甲酯PMMA骨水泥对SD仔鼠原代成骨细胞的增殖以及凋亡的影响。方法：采用分离SD仔鼠颅骨与胰酶消化法获取SD仔鼠原代成骨细胞，通过细胞爬片碱性磷酸酶染色对原代成骨细胞进行鉴定；利用CCK-8法检测低浓度万古霉素复合PMMA及纯PMMA骨水泥浸提液对SD大鼠原代成骨细胞的增殖影响、流式细胞仪检测万古霉素复合PMMA及纯PMMA骨水泥浸提液对对SD大鼠原代成骨细胞的凋亡影响。结果：SD大鼠原代成骨细胞在低浓度万古霉素复合PMMA骨水泥浸提液中第1、2、3天的增殖更明显(P < 0.05)，在纯PMMA骨水泥浸提液中第1、5天凋亡显著增加(P < 0.05)。结论：低浓度万古霉素复合PMMA骨水泥相比PMMA骨水泥具有较低的细胞毒性，在临床应用于骨缺损伴局部骨感染的治疗方面具有一定的优势。 |
| 英文摘要: |
| ABSTRACT Objective: To study the cytotoxicity of vancomycin (low concentration,1%)/polymethyl methacrylate(PMMA) cements on SD rat-derived primary osteoblasts. Methods: Primary osteoblasts were obtained by the calvarias trypsin-digestion method, which were isolated from newborn Sprague-Dawley rats. To identify the primary osteoblasts, alkaline phosphatase staining was used.The activity of cell proliferation in vancomycin (low concentration)/ polymethyl methacrylate (PMMA) and polymethyl methacrylate (PMMA) cements extract were assessed by CCK-8 assay. Apoptosis of osteoblasts induced by vancomycin (low concentration)/ polymethyl methacrylate (PMMA) and polymethyl methacrylate (PMMA) cements extract was observed by flow cytometry. Results: The activity of SD rat rat-derived osteoblasts proliferation in the vancomycin (low concentration)/ polymethyl methacrylate (PMMA)cement extracts was significantly more than that in the group of PMMA cement extracts on day 1, 2, 3(P < 0.05). The apoptotic rate of primary osteoblasts in extracts from the vancomycin (low concentration)/PMMA cements was significantly lower than that in extract from the PMMA cement on day 1 and 5(P < 0.05). Conclusion: Compared with PMMA cements, the vancomycin (low concentration)/PMMA cements has a good cytocompatibility with primary osteoblasts. It will be useful inbone defect with infection repair. |
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