| 易忠权,夏 伟,赵盼雯,崔玉宝.DNA标记技术在螨类系统学研究中的应用[J].现代生物医学进展英文版,2017,17(31):6196-6200. |
| DNA标记技术在螨类系统学研究中的应用 |
| DNA Markers in the Systematic Study of Mites |
| Received:December 28, 2016 Revised:January 23, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.31.046 |
| 中文关键词: DNA标记 螨类 RAPD RFLP SSR 核酸序列分析 AFLP DALP |
| 英文关键词: DNA markers mites RAPD RFLP SSR DNA Sequence Analysis AFLP DALP |
| 基金项目:国家自然科学基金项目（NSFC31572319） |
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| 中文摘要: |
| 摘要：长期以来，螨类主要依靠其形态特征进行系统学研究。DNA标记是指能反映生物个体或物种间基因组中某种差异特征的DNA片段。近年来，DNA标记技术在螨类系统学研究中得到越来越广泛的应用。本文综述了随机扩增多态性RAPD、限制性内切酶片段长度多态性RFLP、微卫星SSR、核酸序列扩增、扩增片段长度多态性AFLP和直接扩增片段长度多态性DALP 等6种DNA标记技术在螨类系统学研究中的应用现状及前景。 |
| 英文摘要: |
| ABSTRACT: For a long time, mites mainly rely on the morphological characteristics in the systematic study. DNA markers are DNA fragments which can reflect the characteristics of the genome between individual species or species. Recently, the application of DNA markers has become more and more important in the systematic study of mites. The present situation and prospect of DNA mark- ers, RAPD (Random Amplified Polymorphic), RFLP (Restriction Fragment Length Polymorphism), SSR (Simple Sequence Repeat), DNA Sequence Analysis, AFLP (Amplified Fragment Length Polymorphism) and DALP (Direct Amplification of Length Polymor- phism), used in the systematic study of mites are described here, which will be helpful for research on acarology. |
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