| 段晓丹,于建平,李 娜,张 梅,王军锋.携带TSST-1和PVL基因的金黄色葡萄球菌的耐药特点、分布特征及与致病性的关系[J].现代生物医学进展英文版,2017,17(31):6179-6182. |
| 携带TSST-1和PVL基因的金黄色葡萄球菌的耐药特点、分布特征及与致病性的关系 |
| Resistant Characteristics, Distribution Characteristics and Correlation with the Pathogenicity of Staphylococcus Aureus withTSST-1 and PVL |
| Received:May 08, 2017 Revised:May 29, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.31.042 |
| 中文关键词: 中毒休克综合征毒素-1 杀白细胞毒素 金黄色葡萄球菌 抗菌药物 耐药性 |
| 英文关键词: Toxic shock syndrome toxin -1 Cytotoxicity Staphylococcus aureus Antimicrobial agents Drug resistance |
| 基金项目: |
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| 中文摘要: |
| 摘要 目的：探讨携带中毒休克综合征毒素-1(TSST-1)和杀白细胞毒素(PVL)基因的金黄色葡萄球菌的耐药特点、分布特征及其与致病性的关系。方法：收集金黄色葡萄球菌临床分离菌株93株，采用聚合酶链反应(PCR)检测TSST-1基因和PVL基因，采用琼脂扩散法检测金黄色葡萄球菌菌株对青霉素(PEN)、苯唑西林(OXA)、头孢噻吩(CEF)、氨苄西林(AMP)、头孢噻肟(CTX)、阿莫西林/克拉维酸(AMC)、亚胺培南(IPM)、克拉霉素(CLR)、万古霉素(VAN)、环丙沙星(CIP)、庆大霉素(GM)、左氧氟沙星(LVX)和利福平(RA)13种抗菌药物的耐药性。结果：耐甲氧西林金黄色葡萄球菌(MRSA)占总数的88.2%，甲氧西林敏感金黄色葡萄球菌(MSSA)占总数的11.8%。TSST-1+菌株在MRSA、MSSA中分别占12.2%、0，差异无统计学意义(P＞0.05)；PVL+菌株在MRSA、MSSA中分别占40.2%、9.1%，差异有统计学意义(P＜0.05)。MRSA存在明显的耐药性，且表现出多药耐药性，而携带TSST-1与PVL基因的MRSA耐药性更严重。结论：MRSA在金黄色葡萄球菌中的分离率高，耐药性严重，携带TSST-1与PVL基因的MRSA耐药性与致病力增加。 |
| 英文摘要: |
| ABSTRACT Objective: To discuss the resistant characteristics, distribution characteristics and correlation with the pathogenicity of Staphylococcus aureus with toxic shock syndrome toxin-1 (TSST-1) and Panton-Valentine leukocidin (PVL). Methods: Staphylococcus aureus (93 strains) of clinical isolated were collected, and the TSST-1 gene and PVL gene were detected by Polymerase chain reaction (PCR). The resistant characteristics of Staphylococcus aureus to penicillin (PEN), benzene azole Westwood (OXA), cefalotin (CEF), ampicillin (AMP), cefotaxime (CTX), amoxicillin/clavulanic acid (AMC), imipenem (IPM), clarithromycin (CLR), vancomycin (VAN), ciprofloxacin (CIP), gentamicin (GM), levofloxacin (LVX) and rifampicin (RA) were detected by agar diffusion method. Results: The methicillin-resistant staphylococcus aureus (MRSA) was accounted for 88.2% and methicillin sensitive staphylococcus aureus (MSSA) was accounted for 11.8%, the two data had significant difference(P＜0.05); The TSST-1+ strains of MRSA and MSSA were accounted for 12.2% and 0, the two data had no significant difference(P＜0.05); The PVL+ strains of MRSA and MSSA were accounted for 40.2% and 9.1%, the two data had significant difference(P＜0.05). MRSA showed obvious resistance and multi-drug resistance, while the MRSA with TSST-1and PVL showed more serious resistance. Conclusion: The separation rate was high in staphylococcus aureus and showed serious resistance. And the resistance and pathogenicity of MRSA with TSST-1and PVL were further increased. |
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