| 左一雄,蔡湘仪,吴彦萍,黄来强.山萘酚对人非小细胞肺癌细胞系A549增殖的影响[J].现代生物医学进展英文版,2017,17(31):6001-6005. |
| 山萘酚对人非小细胞肺癌细胞系A549增殖的影响 |
| The Effect of Kaempferol on the Proliferation of Human Alveolar Adenocarcinoma Cell Line A549 |
| Received:March 21, 2017 Revised:April 11, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.31.001 |
| 中文关键词: 山萘酚 A549细胞 上游结合因子 增殖 |
| 英文关键词: Kaempferol A549 Cell Upstream Binding Factor Proliferation |
| 基金项目:国家自然科学基金项目(30570960)；深圳市科技计划项目(JCYJ20140418112611757, GJHZ20140416153844269) |
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| 中文摘要: |
| 摘要 目的：探究山萘酚(Kaempferol，Kpf)对非小细胞肺癌细胞系A549增殖的影响。方法：采用不同浓度(0、5、10和20 μg?mL-1)的山萘酚处理体外培养的A549细胞后，通过四氮唑蓝(MTT)试验和平板克隆试验探究山萘酚对A549增殖的影响，实时定量聚合酶链式反应(Q-PCR)来探究山萘酚作用下rDNA的转录情况，免疫印迹试验探究山萘酚对上游结合因子(Upstream binding factor，UBF)磷酸化的影响。结果：MTT和平板克隆试验证明山萘酚对A549细胞的增殖有较强的抑制作用，存在剂量依赖关系。Q-PCR证明了山萘酚能够下调rDNA的转录。通过免疫印迹试验能够证明山萘酚可以降低UBF磷酸化的水平。结论：山萘酚对人非小细胞肺癌细胞系A549的生长有抑制作用，可能与下调UBF的磷酸化水平影响rDNA的转录有关。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect of Kaempferol on the proliferation of human alveolar adenocarcinoma cell line A549. Methods: A549 cells was treated with different concentrations of Kaempferol(0, 5, 10, 20, 50 μg?mL-1). MTT assay and plate cloning ex- periments were contributed to study the effect of Kaempferol on the cell proliferation. The nucleolus transcription level was detected by real-time polymerase reaction experiment. Western blot was performed to detect the phosphorylation of upstream binding factor (UBF) protein. Results: MTT assay and plate cloning experiments showed that Kaempferol significantly decreased the viability of A549 cells in a dose dependent manner. Moreover, Keampferol down-regulated the level of nucleolus transcription, declined the phosphorylated UBF protein level. Conclusion: Keampferol could inhibit the proliferation of A549 cells, which might be related to the down-regulation of pre-rRNA and p-UBF protein level. |
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