| 赵 远,赵 伟,王象勇,任卫全,赵久兴.亚甲蓝光化学法灭活新鲜冰冻血浆病毒及其效果评价[J].现代生物医学进展英文版,2017,17(30):5955-5958. |
| 亚甲蓝光化学法灭活新鲜冰冻血浆病毒及其效果评价 |
| Fresh Frozen Plasma Virus Inactivated by Methylene Blue Photochemistry and Its Effect Assessment |
| Received:May 07, 2017 Revised:May 30, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.30.035 |
| 中文关键词: 亚甲蓝光化学法 新鲜冰冻血浆 病毒 灭活 效果评价 |
| 英文关键词: Methylene blue photochemistry Fresh frozen plasma Virus Inactivation Effect assessment |
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| 中文摘要: |
| 摘要 目的：探讨以亚甲蓝光化学法(MB-P)灭活新鲜冰冻血浆病毒及其效果。方法：选取2013年3月-2016年11月来青海省血液中心无偿献血者捐献的400 mL全血共1500份，分离新鲜冰冻血浆200 mL后，再分为两份即对照组和观察组各100 mL，各1500份。对照组不经灭活，实验组经MB-P病毒灭活。比较两组的纤维蛋白原(FIB)、总蛋白(TP)、凝血因子VIII(FVIII)及白细胞(WBC)的含量。再随机抽取500例接受了上述病毒灭活血浆输注的患者，观察其输血反应的发生情况。结果：观察组的FIB和TP含量及FVIIII活性比对照组有所降低，但差异不具有统计学意义(P＞0.05)；观察组的WBC含量较对照组明显降低，且具有统计学差异(P＜0.05)；FIB、TP、FVIIII的回收率的分别为85.43%、91.08%和80.49%，WBC的残留率为0.21%；500例接受了上述病毒灭活血浆输注的患者中，有1例出现发热症状。结论：经MB-P灭活病毒血浆的有效成分含量较高，符合国家相关标准，并且WBC含量和输血不良反应发生率均较低，满足临床安全输血的要求，但仍需严格要求血液收集和血浆病毒灭活的操作过程并严格掌握输血适应症，避免人为造成的输血风险。 |
| 英文摘要: |
| ABSTRACT Objective: To discuss the fresh frozen plasma virus inactivated by methylene blue photochemistry (MB-P) and its ef- fect assessment. Methods: The 1500 copies (400 mL/copy) whole blood donated by unpaid blood donors of Blood Center from Qinghai Province in March 2013 to November 2016 were selected. The fresh frozen plasma was separated about 200 mL from whole blood and furtherly divided into two copies of control group and observation group each 100 mL. The control group was no inactivated, while ex- perimental group was virus inactivated by MB-P. The fibrinogen (FIB), total protein (TP), blood coagulation factor VIII (FVIII) and the content of white blood cells (WBC) of two groups were compared. The blood transfusion reactions of patients accepted the virus inacti- vated plasma infusion (500 cases) were observed. Results: The FIB, TP and FVIII of observation group decreased less than control group, but that had no statistically significant difference (P＞0.05); The content of WBC of observation group was significantly lower than that of control group (P＜0.05); The recovery rates of fibrinogen, total protein and blood coagulation factor VII were 85.43%, 91.08% and 80.49%. And the residual rate of white blood cells was 0.21%; There was 1 case showing fever symptom in 500 cases patients accepted the virus inactivated plasma infusion. Conclusion: The effective composition content of plasma viruses inactivated by MB-P is high and complis with the relevant national standards. Besides, WBC level and incidence of adverse reactions are both and to meet the require- ments of the clinical safety of blood transfusion. However, blood collection operation process, blood plasma virus inactivation operation process and the blood transfusion indications master should be strictly required to avoid man-made blood transfusion risk. |
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