| 孙 慧,尹兴平,蒋屏东,倪菁菁,张瑞丽.皮肤性病患者人乳头瘤病毒基因分型检测的临床分析[J].现代生物医学进展英文版,2017,17(30):5879-5882. |
| 皮肤性病患者人乳头瘤病毒基因分型检测的临床分析 |
| Clinical Analysis of Human Papillomavirus Genotyping in Patients with Skin and Venereal Diseases |
| Received:March 28, 2017 Revised:April 20, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.30.018 |
| 中文关键词: 人乳头瘤病毒 基因分型 随访管理 |
| 英文关键词: Human papilloma virus, Genotyping Follow-up management |
| 基金项目:江苏省自然科学基金项目(BK20150121) |
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| 中文摘要: |
| 摘要 目的：了解皮肤性病患者不同病变类型中的HPV感染型别。方法：选择2014年6月～2015年6月我科门诊就诊者368例，分为4个组别：尖锐湿疣患者组242例，鲍温样丘疹病患者18例，男性冠状沟珍珠疹和女性假性湿疣70例，未见任何皮疹且醋酸白试验阴性的体检者38例。采用PCR-反向点杂交法检测皮损或外阴局部HPV-DNA亚型，并用SPSS11.0软件进行统计学分析。结果：(1)HPV-DNA的总检出率为72.9 %，其中单一型别感染率57.1 %，多重感染率15.8 %；(2)268例HPV阳性标本中，高危型感染占51.9 %，低危型和混合型的阳性率分别为33.2 %、14.9 %；(3)尖锐湿疣组和鲍温样丘疹病患者组HPV-DNA的阳性率分别为97.9 %、88.9 %，而男性珍珠疹和女性假性湿疣组以及要求体检人群的阳性率分别为14.3 %和13.2 %；从感染型别分析，尖锐湿疣主要是6、11、16、18、31、33、35、43和66亚型，鲍温样丘疹病患者主要是16亚型，男性珍珠疹和女性假性湿疣以及要求体检人群的感染型别主要是低危型感染，分别是6、42、43、81和6、42、83；(4)在被检测的18个高危HPV亚型中，最常见类型依次为HPVl6、18、58、56、33、52、68、31、39，未检测出HPV35、45、51、53、59、66、73和82亚型；在被检测的5个低危HPV-DNA亚型中依次为HPV6、11、42和43，未检测出81亚型。结论：HPV感染以单一型别感染为主，且以高危型为主，应该重视临床HPV感染亚型的检测，尤其是高危型HPV感染者的随访管理。 |
| 英文摘要: |
| ABSTRACT Objective: To understand the subtypes of HPV infection in different skin diseases. Methods: 368 patient were included in the study from June 2014 to June 2015 and divided into four groups: 242 cases with condyloma acuminatagroup, 18 cases with Bowenoid papulosis, 70 cases with male pearl rash and female pseudo-condyloma, 38 cases without any visible lesions and acetowhite test negative. The HPV -DNA subtypes in the clinical samples were examined by reverse dot hybridization combined with PCR. All the data were statistically analyzed using SPSS11.0 software. Results: (1) The total positive rate of HPV was 72.9 %, with the single type 57.1 % and multiple types 15.8 %. (2) Of all the 268 cases with HPV infection, the infection rate by the high-risk type of HPV virus was 51.9 % and those of the low-risk and mixed types 33.2 %, 14.9 %, respectively. (3) The infection rates in different groups are as follows, condyloma acuminatagroup 97.9 %, bowenoid papulosis 88.9 %, male pearl rash and female pseudo-condyloma14.3 %, patients without any visible lesions and acetowhite test negative 13.2 %. According to the analysis of infection HPV subtypes, HPV 6, 11, 16, 18, 31, 33, 35, 43 and 66 subtypes, were mainly found in condyloma acuminata group and 16 subtypes in Bowenoid papulosis while male pearl rash and female pseudo-condyloma and others infected mainly by low risk types of 6, 42, 43, 81 and 83. (4) Of the 18 high risk subtypes, the most common types found were HPVl6, 18, 58, 56, 33, 52, 68, 31, 39 but HPV35, 45, 51, 53, 59, 66, 73 and 82 subtypes were not detected. Among the5 low-risk HPV subtypes, HPV6, 11, 42 and 43 were detected but no 81 subtypes. Conclusion: The study showed that HPV infection was mainly single HPV subtype, mostly high-risk type, which should be given much consideration in clinical practice. |
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