赵晓红,冯 丽,张百红,马文英,胡文梅.二甲双胍联合卡铂对三阴性乳腺癌细胞增殖的影响[J].现代生物医学进展英文版,2017,17(30):5837-5839. |
二甲双胍联合卡铂对三阴性乳腺癌细胞增殖的影响 |
Effects of Metformin combined with Carboplatin on the Proliferation of Triple Negative Breast Cancer Cells |
Received:April 27, 2017 Revised:May 20, 2017 |
DOI:10.13241/j.cnki.pmb.2017.30.008 |
中文关键词: 二甲双胍 卡铂 三阴性乳腺癌细胞 细胞增殖 |
英文关键词: Metformin Carboplatin Triple negative breast cancer cell Proliferation |
基金项目:甘肃省自然科学基金项目(1308RJZA181) |
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中文摘要: |
摘要 目的:探讨二甲双胍联合卡铂对三阴性乳腺癌细胞MDA-MB-231增殖的影响。方法:体外培养三阴性乳腺癌细胞MDA-MB-231,分别给予不同浓度(0、2.5、5、10 mmol/L) 二甲双胍和20 μmol/L卡铂处理后,采用MTT实验、平板克隆实验检测二甲双胍联合卡铂对MDA-MB-231细胞增殖的影响。结果:MTT实验结果显示:二甲双胍抑制MDA-MB-231细胞的增殖,5、10 mM组细胞OD490值均显著低于对照组(P <0.05),且10 mM组细胞OD490值明显低于其他各组(P<0.05);与单药相比,二甲双胍和卡铂联用组细胞生长抑制率显著升高,差异具有统计学意义(P<0.05)。平板克隆实验结果显示:与单药相比,二甲双胍和卡铂联用组细胞的克隆形成抑制率率显著升高,差异具有统计学意义(P<0.05)。结论:二甲双胍联合卡铂能够有效的抑制三阴性乳腺癌细胞MDA-MB-231的增殖。 |
英文摘要: |
ABSTRACT Objective: To explore the effect of metformin combined with carboplatin on the proliferation of triple negative breast cancer cells MDA-MB-231. Methods: MDA-MB-231 cell was cultured in vitro and treated with different concentrations (0, 2.5, 5, 10 mmol/L) of metformin and 20 μmol/L carboplatin, respectively. MTT and clone formation assays were used to detect the effect of metformin combined with carboplatin on the proliferation of MDA-MB-231 cells. Results: MTT assay showed that metformin inhibited the proliferation of MDA-MB-231 cells. The cells OD490 values in 5 or 10mM group were significantly lower than that of the control group (P <0.05), and the OD490 values in 10 mM group were significantly lower than those in the other groups (P <0.05). Compared with monotherapy, the cell growth inhibition rate of metformin combined with carboplatin was significantly increased(P<0.05). The results of plate clone formation assay showed that compared with monotherapy, the cell clone formation inhibition rate of metformin combined with carboplatin was significantly increased(P<0.05). Conclusion: Metformin combined with carboplatin could effectively reduce the proliferation of triple negative breast cancer cell line MDA-MB-231. |
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