| 任婧婧,李 光,刘志勤,张韵洁,谢 佳,刘 锋,戴进前,宋艳萍.RNA干扰Mcl-1基因表达对淋巴瘤Raji细胞增殖和凋亡的机制研究[J].现代生物医学进展英文版,2017,17(30):5823-5826. |
| RNA干扰Mcl-1基因表达对淋巴瘤Raji细胞增殖和凋亡的机制研究 |
| The Mechanism of RNA Interference Mcl-1 Gene Expression on the Proliferation and Apoptosis of Lymphoma Raji Cells |
| Received:April 28, 2017 Revised:May 25, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.30.005 |
| 中文关键词: 淋巴瘤 Mcl-1基因 增殖 凋亡 RNA干扰 Notch1信号通路 |
| 英文关键词: Lymphoma Mcl-1 gene Proliferation Apoptosis RNA interference Notch1 signaling pathway |
| 基金项目:陕西省科学技术研究发展计划项目（2014K11-02-03-03） |
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| 中文摘要: |
| 摘要 目的：探讨抑制Mcl-1基因表达对淋巴瘤Raji细胞增殖和凋亡的影响及机制。方法：NC-siRNA、Mcl-1-siRNA转染Raji细胞，以不作任何处理的细胞作为空白对照组，48h后Western blot检测各组细胞中Mcl-1的蛋白表达；CCK8实验和流式细胞仪分别检测细胞的增殖和凋亡情况；Western blot检测Cleaved caspase3、Notch1、Hes1蛋白表达。结果：转染Mcl-1-siRNA后Mcl-1的蛋白表达显著降低；与对照组及NC-siRNA组比较，Mcl-1-siRNA组细胞存活率显著降低，细胞凋亡率显著升高，Cleaved caspase3蛋白显著上调表达，Notch1和Hes1蛋白显著下调表达。结论：RNA干扰抑制Mcl-1基因表达可显著降低Raji细胞增殖及诱导细胞凋亡，其机制与抑制Notch1信号通路有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect and mechanism of RNA interference Mcl-1 gene expression on proliferation and apoptosis of lymphoma Raji cells. Methods: NC-siRNA and Mcl-1-siRNA were transfected into Raji cells, the cells without any treatment as the control group, the expression of Mcl-1 protein was detected by Western blot after 48h; CCK8 assay and flow cytometry were used to detect the proliferation and apoptosis of the cells; expression of Cleaved Caspase3, Notch1, Hes1 protein were detected by Western blot. Results: Mcl-1 expression after transfected Mcl-1-siRNA was significantly decreased; compared with the control group and NC-siRNA group, cell survival rate in Mcl-1-siRNA group decreased significantly, the apoptosis rate was significantly increased, the expression of Cleaved Caspase3 protein was significantly up-regulated, Notch1 and Hes1 protein were significantly down-regulated. Conclusion: RNA interference can inhibit the expression of Mcl-1 gene and decrease the proliferation and induce apoptosis of Raji cells, which may be related to the inhibition of Notch1 signaling pathway. |
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