| 段秋霞,杨丹丹,彭晓燕,郭菲菲,徐 珞.下丘脑弓状核微量注射Orexin-A对大鼠胃传入信息和胃运动的影响[J].现代生物医学进展英文版,2017,17(29):5628-5632. |
| 下丘脑弓状核微量注射Orexin-A对大鼠胃传入信息和胃运动的影响 |
| The Impact of Stomach Information and Gastric Motility in Microinjection of Orexin-A into the Rat Hypothalamic Arcuate Nucleus |
| Received:March 02, 2017 Revised:March 25, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.29.006 |
| 中文关键词: orexin 弓状核 胃牵张敏感神经元 胃运动 |
| 英文关键词: Orexin Arcuate nucleus Gastric distension responsive neurons Gastric motility |
| 基金项目:国家自然科学基金项目（81470815,81270460,81500414）；山东省优秀中青年科学基金项目（BS2014YY009）；青岛市科技局项目（14-2-3-3-nsh） |
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| 中文摘要: |
| 摘要 目的：探讨ARC orexin-A对胃传入信息以及胃运动的调控及机制。方法：采用细胞外放电记录方法，鉴定ARC orexin胃牵张敏感神经元（Gastric distention sensitive neurons，GD），并探讨ARC 内orexin-A对GD神经元放电活动的影响及机制；采用ARC微量注射orexin-A和及其受体阻断剂SB334867，观察大鼠胃收缩幅度和频率的改变。结果：大鼠ARC共记录到149 个GD神经元，其中GD-E神经元91 个，GD-I神经元58 个。ARC微量注射orexin-A，62 个（62/91，68.1%）GD-E神经元兴奋性显著增加，其放电频率由4.27±0.58 Hz 增加到8.46±0.95 Hz（P<0.01）；39 个（39/58，67.2%）GD-I神经元兴奋性也显著增强，其放电频率由 4.02±0.53 Hz 增加到5.43±0.57 Hz（P<0.05）。然而，ARC给予大鼠orexin-A受体拮抗剂SB334867，再给予orexin-A，orexin-A兴奋效应完全被阻断（P>0.05）。胃运动实验结果显示：在ARC注射不同浓度orexin-A，大约5 min后，大鼠胃收缩幅度和频率呈剂量依赖性增加（P<0.05～0.01）。ARC注射SB334867，可完全消除orexin-A对大鼠胃运动的兴奋效应（P<0.05）。结论：ARC orex- in-A对大鼠GD神经元和胃运动有调控作用，该作用可能通过调控Orexin A受体活动实现的。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the regulation and mechanism of ARC orexin-A on gastric afferent information and gastric motility. Methods: The extracellular discharge recording method: identification of ARC orexin gastric stretch sensitive neurons (Gastric distention, sensitive neurons, GD), and to explore the effect of orexin-A on ARC activity of GD neurons and its mechanism; antagonist SB334867 by ARC microinjection of orexin-A and its receptor, observe the rat gastric contraction amplitude and frequency change. Results: A total of 149 GD neurons were recorded in ARC rats, of which GD-E neurons were and GD-I neurons were 58. ARC microin- jection of orexin-A, 62 (62/91, 68.1%) significantly increased the excitability of GD-E neurons, the discharge frequency from 4.27±0.58 Hz to 8.46±0.95 Hz (P<0.01); 39 (39/58, 67.2%) the excitability of GD-I neurons also increased significantly, the discharge frequency from 4.02±0.53 Hz to 5.43±0.57 Hz(P<0.05). However, ARC was given to rat orexin-A receptor antagonist SB334867, and then given orexin-A, the orexin-A effect was completely blocked (P>0.05). The results of gastric motility test showed that the amplitude and fre- quency of gastric contraction in rats were increased in a dose-dependent manner (P<0.05 ~ 0.01) after ARC injection at different concen- trations of orexin-A (about 5 min). The effect of orexin-A on gastric motility in rats was completely eliminated by ARC injection of SB334867 (P<0.05). Conclusion: ARC orexin-A may regulate the activity of GD neurons and gastric motility in rats, which may be medi- ated by the regulation of Orexin A receptor activity. |
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