| 张 波,曾帮智,张 博,张 茜,吕晓玥.胃癌组织中p53、p21WAF1蛋白的表达及与幽门螺杆菌L型感染的关系研究[J].现代生物医学进展英文版,2017,17(28):5569-5573. |
| 胃癌组织中p53、p21WAF1蛋白的表达及与幽门螺杆菌L型感染的关系研究 |
| Expression of p53 and p21WAF1 Proteins in Gastric Carcinoma and Their Relationship with Helicobacter Pylori L-type Infection |
| Received:May 01, 2017 Revised:May 30, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.28.039 |
| 中文关键词: p53 p21WAF1 Hp-L型感染 相关性 |
| 英文关键词: p53 p21WAF1 Hp-L infection Correlation |
| 基金项目: |
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| 中文摘要: |
| 摘要 目的：研究胃癌组织中磷蛋白53（P53）、磷蛋白21野生型p53活化片段(p21WAF1)蛋白的表达及与幽门螺杆菌L型(Hp-L)感染的关系。方法：选择我院2013年1月至2017年1月病理确诊为胃癌的标本220例为胃癌组，另外选取胃癌组织旁的健康组织为对照组。Hp-L采用快速尿素酶测试法、Giemsa染色法检测，P53、p21WAF1阳性表达采用HE法及免疫组化法测定，并采用Spearman等级检验分析其相关性。结果：胃癌组p53阳性表达率显著高于对照组(P<0.05)；p21WAF1阳性表达率显著低于对照组(P<0.05)，胃癌组织中p53和p21WAF1表达呈负相关（P<0.05）。胃癌组Hp-L阳性感染率显著高于对照组(P<0.05)；胃癌组Hp-L阳性组p53阳性表达率显著高于于阴性组(P<0.05)；p21WAF1阳性表达率显著低于阴性组(P<0.05)，胃癌组织中p53与Hp-L阳性表达呈正相关(P<0.05)，p21WAF1与Hp-L阳性表达呈负相关(P<0.05)， p53阳性表达仅与浸润程度有关(P<0.05)，P21WAF1表达与病理分级、浸润程度、UICC分期有关(P<0.05)。结论：Hp-L是胃癌发生的主要诱因，癌症发展过程中，Hp-L型感染可上调p53表达、抑制p21WAF1表达，且p53、p21WAF1的表达变化与细胞浸润程度有密切联系，对胃癌的临床治疗具有重要作用。 |
| 英文摘要: |
| ABSTRACT Objective: To study the expression of proteina fosforilata 53（p53）and proteina fosforilata 21 Wide-type actiated factorlp（p21WAF1） protein in gastric carcinoma and its relationship with Helicobacter pylori-L（Hp-L）. Methods: 220 patients with gastric cancer were confirmed by pathology in our hospital from January 2013 to January 2017 were selected as gastric cancer group. The healthy tissues of gastric cancer tissues were taken as the control group. The positive expression of Hp-L was detected by rapid urease test and Giemsa staining. The positive expression of P53 and p21WAF1 were detected by HE and immunohistochemistry, the correlation was analyzed by Spearman rank test. Results: The positive expression rate of p53 in gastric cancer group was significantly higher than that in control group(P<0.05). The positive expression of p21WAF1 was significantly lower than that of the control group(P<0.05), there was a negative correlation between p53 and p21WAF1 expression in gastric carcinoma (P<0.05); The positive rate of Hp-L positive in gastric cancer group was significantly higher than that in control group(P<0.05). The positive expression of p53 in Hp-L positive group was significantly higher than that in negative group(P<0.05). The positive expression of p21WAF1 was significantly lower than that of negative group(P<0.05). There was a positive correlation between p53 and Hp-L positive expression(P<0.05), and p21WAF1 was negatively correlated with Hp-L expression(P<0.05). The positive expression of p53 was only correlated with the degree of invasion (P<0.05), the expression of P21WAF1 was correlated with pathological grade, infiltration degree and UICC stage (P<0.05). Conclusion: Hp-L is the main cause of gastric cancer, in the process of cancer development, Hp-L infection can up regulate the expression of p53 and inhibit the expression of p21WAF1, the expression of p53 and p21WAF1 is closely related to the degree of cell infiltration, which play an important role in the clinical treatment of gastric cancer. |
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